Mascot Search Results

User            : 
Email           : jpuddick@waikato.ac.nz
Search title    : 
Database        : NCBInr 20090718 (9308017 sequences; 3189511245 residues)
Taxonomy        : Bacteria (Eubacteria) (4990342 sequences)
Timestamp       : 23 Jul 2009 at 22:34:00 GMT
Top Score       : 79 for gi|1065148, Chain A, Barnase A43cS80C DISULFIDE MUTANT

Probability Based Mowse Score

Protein score is -10*Log(P), where P is the probability that the observed match is a random event.
Protein scores greater than 79 are significant (p<0.05).

Score Distribution

Concise Protein Summary Report

Help
  Significance threshold p< Max. number of hits

  

1.    gi|1065148    Mass: 12423    Score: 79     Expect: 0.06  Queries matched: 6
 Chain A, Barnase A43cS80C DISULFIDE MUTANT
      gi|4139770    Mass: 12306    Score: 61     Expect: 3.7  Queries matched: 4
 Chain A, Deletion Of A Buried Salt-Bridge In Barnase
      gi|226887701    Mass: 12478    Score: 60     Expect: 5.3  Queries matched: 4
 Chain A, A Conformationally Strained, Circular Permutant Of Barnase
      gi|230301    Mass: 12375    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Crystal Structure Of A Barnase-D(GpC) Complex At 1.9 Angstroms Resolution
      gi|1065202    Mass: 12333    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Barnase Mutant With Leu 14 Replaced By Ala
      gi|493893    Mass: 12375    Score: 60     Expect: 5.5  Queries matched: 4
 Chain L, Subsite Binding In An Rnase: Structure Of A Barnase- Tetranucleotide Complex At 1.76 Angstroms Resolution
      gi|442654    Mass: 12359    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, The Contribution Of Buried Hydrogen Bonds To Protein Stability: The Crystal Structures Of Two Barnase Mutants
      gi|493902    Mass: 12361    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
      gi|576011    Mass: 12345    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Structural Studies Of Barnase Mutants
      gi|1065209    Mass: 12333    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Barnase Mutant With Ile 76 Replaced By Ala
      gi|66360399    Mass: 12318    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Water-Mediate Interaction At Aprotein-Protein Interface
      gi|253722854    Mass: 12415    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Nmr Structure Of 15n-Labeled Barnase
      gi|576021    Mass: 12142    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Crystallographic Analysis Of Phe->leu Substitution In The Hydrophobic Core Of Barnase
      gi|99032161    Mass: 12176    Score: 60     Expect: 5.5  Queries matched: 4
 Chain A, Barnase Cross-Linked With Glutaraldehyde
      gi|551694    Mass: 16588    Score: 54     Expect: 21  Queries matched: 4
 barnase (RNase) precursor [Bacillus amyloliquefaciens]
      gi|133149    Mass: 17462    Score: 54     Expect: 22  Queries matched: 4
 RecName: Full=Ribonuclease; AltName: Full=Barnase; AltName: Full=RNase Ba; Flags: Precursor
      gi|4139773    Mass: 12305    Score: 43     Expect: 2.4e+02  Queries matched: 3
 Chain A, Deletion Of A Buried Salt Bridge In Barnase
      gi|4139683    Mass: 12318    Score: 42     Expect: 2.8e+02  Queries matched: 3
 Chain A, Structural Response To Mutation At A Protein-Protein Interface


2.    gi|227547853    Mass: 37251    Score: 53     Expect: 27  Queries matched: 4
 fructose-bisphosphate aldolase [Corynebacterium lipophiloflavum DSM 44291]

3.    gi|188990813    Mass: 21716    Score: 51     Expect: 41  Queries matched: 3
 Putative secreted protein [Xanthomonas campestris pv. campestris str. B100]

4.    gi|241891993    Mass: 38358    Score: 48     Expect: 70  Queries matched: 4
 possible altronate dehydratase [Sphingobacterium spiritivorum ATCC 33861]

5.    gi|222084359    Mass: 35757    Score: 48     Expect: 81  Queries matched: 4
 hypothetical protein Arad_0219 [Agrobacterium radiobacter K84]

6.    gi|29832491    Mass: 63005    Score: 48     Expect: 83  Queries matched: 4
 NH(3)-dependent NAD(+) synthetase [Streptomyces avermitilis MA-4680]
      gi|111017934    Mass: 26553    Score: 47     Expect: 1.1e+02  Queries matched: 3
 short chain dehydrogenase [Rhodococcus jostii RHA1]
      gi|228904659    Mass: 4699     Score: 43     Expect: 2.5e+02  Queries matched: 2
 hypothetical protein bthur0014_57890 [Bacillus thuringiensis IBL 4222]
      gi|83644447    Mass: 5364     Score: 42     Expect: 3.2e+02  Queries matched: 2
 hypothetical protein HCH_01600 [Hahella chejuensis KCTC 2396]

7.    gi|56459848    Mass: 62049    Score: 48     Expect: 85  Queries matched: 5
 gamma-glutamyltranspeptidase [Idiomarina loihiensis L2TR]
      gi|117920378    Mass: 20044    Score: 47     Expect: 95  Queries matched: 3
 NUDIX hydrolase [Shewanella sp. ANA-3]

8.    gi|1149710    Mass: 15024    Score: 47     Expect: 1e+02  Queries matched: 3
 tkt [Clostridium perfringens]
      gi|56697006    Mass: 23470    Score: 42     Expect: 3.1e+02  Queries matched: 3
 pyridoxamine 5''-phosphate oxidase, putative [Ruegeria pomeroyi DSS-3]

9.    gi|212637491    Mass: 57024    Score: 46     Expect: 1.4e+02  Queries matched: 4
 Cell division transporter substrate-binding protein FtsY [Shewanella piezotolerans WP3]
      gi|46908614    Mass: 20892    Score: 45     Expect: 1.5e+02  Queries matched: 3
 hypothetical protein LMOf2365_2414 [Listeria monocytogenes str. 4b F2365]
      gi|226224989    Mass: 20922    Score: 45     Expect: 1.5e+02  Queries matched: 3
 hypothetical protein Lm4b_02410 [Listeria monocytogenes Clip81459]

10.   gi|227410033    Mass: 108378   Score: 45     Expect: 1.6e+02  Queries matched: 6
 anaerobic dehydrogenase, typically selenocysteine-containing [Eggerthella lenta DSM 2243]
      gi|167854816    Mass: 23847    Score: 43     Expect: 2.4e+02  Queries matched: 3
 dethiobiotin synthetase 2 [Haemophilus parasuis 29755]
      gi|213969503    Mass: 26711    Score: 42     Expect: 2.8e+02  Queries matched: 3
 hypothetical protein PSPTOT1_2779 [Pseudomonas syringae pv. tomato T1]

11.   gi|149377837    Mass: 20447    Score: 45     Expect: 1.7e+02  Queries matched: 3
 probable glycosyl hydrolase [Marinobacter algicola DG893]

12.   gi|167718604    Mass: 5090     Score: 45     Expect: 1.7e+02  Queries matched: 2
 aldehyde dehydrogenase (NAD) family protein [Burkholderia pseudomallei DM98]

13.   gi|229169578    Mass: 21244    Score: 44     Expect: 1.9e+02  Queries matched: 3
 hypothetical protein bcere0007_45250 [Bacillus cereus AH621]
      gi|237799599    Mass: 23281    Score: 42     Expect: 3.1e+02  Queries matched: 3
 Rhs element Vgr protein [Pseudomonas syringae pv. oryzae str. 1_6]

14.   gi|92114973    Mass: 23939    Score: 44     Expect: 1.9e+02  Queries matched: 3
 hypothetical protein Csal_2856 [Chromohalobacter salexigens DSM 3043]

15.   gi|195952759    Mass: 26213    Score: 44     Expect: 2.2e+02  Queries matched: 3
 two component transcriptional regulator, winged helix family [Hydrogenobaculum sp. Y04AAS1]

16.   gi|238917766    Mass: 51593    Score: 43     Expect: 2.4e+02  Queries matched: 4
 glycoside/pentoside/hexuronide:cation symporter, GPH family [Eubacterium eligens ATCC 27750]

17.   gi|23014580    Mass: 30841    Score: 43     Expect: 2.6e+02  Queries matched: 3
 hypothetical protein Magn03009023 [Magnetospirillum magnetotacticum MS-1]

18.   gi|60683553    Mass: 122848   Score: 43     Expect: 2.7e+02  Queries matched: 5
 putative outer membrane protein [Bacteroides fragilis NCTC 9343]

19.   gi|227383482    Mass: 25298    Score: 43     Expect: 2.7e+02  Queries matched: 3
 Mn-dependent transcriptional regulator [Jonesia denitrificans DSM 20603]

20.   gi|197775281    Mass: 21160    Score: 43     Expect: 2.7e+02  Queries matched: 3
 transcriptional regulator [Streptomyces pristinaespiralis ATCC 25486]

Search Parameters

Type of search         : Peptide Mass Fingerprint
Enzyme                 : Trypsin
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 200 ppm
Peptide Charge State   : 1+
Max Missed Cleavages   : 1
Number of queries      : 39
Selected for scoring   : 12

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