Mascot Search Results

User            : 
Email           : jpuddick@waikato.ac.nz
Search title    : 
MS data file    : DATA.TXT
Database        : NCBInr 20090718 (9308017 sequences; 3189511245 residues)
Taxonomy        : Bacteria (Eubacteria) (4990342 sequences)
Timestamp       : 20 Jul 2009 at 03:56:34 GMT
Protein hits    : gi|133245 RecName: Full=Ribonuclease; AltName: Full=Binase; AltName: Full=RNase Bi; Flags: Precursor
  gi|493911 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
  gi|493905 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
  gi|229541855 ABC transporter related [Bacillus coagulans 36D1]
  gi|229876264 predicted GTPase, G3E family [Sphaerobacter thermophilus DSM 20745]
  gi|53712057 GTP pyrophosphokinase [Bacteroides fragilis YCH46]
  gi|223038984 endonuclease IV [Campylobacter rectus RM3267]
  gi|168335189 amidotransferase HisH [Epulopiscium sp. 'N.t. morphotype B']
  gi|224373298 metal dependent phosphohydrolase [Nautilia profundicola AmH]
  gi|29349483 hypothetical protein BT_4075 [Bacteroides thetaiotaomicron VPI-5482]

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 50 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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Overview Table

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Hit: 1 2 3 4 5 6 7 8 9 10
1386.7469 (1+)

        Error tolerant   

1.    gi|133245    Mass: 17945    Score: 40     Queries matched: 1
 RecName: Full=Ribonuclease; AltName: Full=Binase; AltName: Full=RNase Bi; Flags: Precursor
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.7231   11.9 0  40  0.51 1   R.LVYSSDWLIYK.T

 
      Proteins matching the same set of peptides:
      gi|585908    Mass: 12174    Score: 40     Queries matched: 1
 RecName: Full=Ribonuclease; AltName: Full=RNase Bco
      gi|1350819    Mass: 17903    Score: 40     Queries matched: 1
 RecName: Full=Ribonuclease; AltName: Full=RNase Bp; Flags: Precursor
      gi|1942120    Mass: 12181    Score: 40     Queries matched: 1
 Chain A, Structure Of Binase Mutant His 101 Asn
      gi|17942905    Mass: 12950    Score: 40     Queries matched: 1
 Chain A, Hydrolase(Endoribonuclease)ribonuclease Bi (G Specific Endonuclease) (E.C.3.1.27.-) Complexed With Guanosine-3'- Phosphate (3'-Gmp)
      gi|157693862    Mass: 17900    Score: 40     Queries matched: 1
 guanine-specific ribonuclease [Bacillus pumilus SAFR-032]
      gi|194015154    Mass: 17899    Score: 40     Queries matched: 1
 ribonuclease (RNase Bp) [Bacillus pumilus ATCC 7061]
      gi|253722754    Mass: 12204    Score: 40     Queries matched: 1
 Chain A, Structure Of Binase In Solution


2.    gi|493911    Mass: 12361    Score: 40     Queries matched: 1
 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.7231   11.9 0  40  0.51 1   R.IVYSSDWLIYK.T


3.    gi|493905    Mass: 12361    Score: 37     Queries matched: 1
 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.7231   11.9 0  37  1.2 3   R.VLYSSDWLIYK.T


4.    gi|229541855    Mass: 32661    Score: 25     Queries matched: 1
 ABC transporter related [Bacillus coagulans 36D1]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.6900   35.8 0  25  17 4   -.MGYALEIEGLYK.S


5.    gi|229876264    Mass: 36486    Score: 22     Queries matched: 1
 predicted GTPase, G3E family [Sphaerobacter thermophilus DSM 20745]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.8282   -63.95 0  22  37 5   R.IPVLIVSGFLGSGK.T


6.    gi|53712057    Mass: 85857    Score: 20     Queries matched: 1
 GTP pyrophosphokinase [Bacteroides fragilis YCH46]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.8322   -66.82 1  20  55 6   K.KLSFLVYIYIK.G

 
      Proteins matching the same set of peptides:
      gi|60680251    Mass: 85842    Score: 20     Queries matched: 1
 putative RelA/SpoT GTP pyrophosphokinase [Bacteroides fragilis NCTC 9343]

7.    gi|223038984    Mass: 72380    Score: 17     Queries matched: 1
 endonuclease IV [Campylobacter rectus RM3267]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.7442   -3.29 1  17  95 7   K.TLSKYEELLYK.D


8.    gi|168335189    Mass: 21949    Score: 17     Queries matched: 1
 amidotransferase HisH [Epulopiscium sp. 'N.t. morphotype B']
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.7278   8.54 1  17  1e+02 8   K.IPHMGWNKLYK.T


9.    gi|224373298    Mass: 96037    Score: 17     Queries matched: 1
 metal dependent phosphohydrolase [Nautilia profundicola AmH]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.7265   9.50 1  17  1e+02 9   K.KMDEFVTLIYK.Y


10.   gi|29349483    Mass: 54412    Score: 17     Queries matched: 1
 hypothetical protein BT_4075 [Bacteroides thetaiotaomicron VPI-5482]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7469   1385.7396   1385.7628   -16.75 1  17  1.1e+02 10   R.YMKIIVDTIYK.M

 
      Proteins matching the same set of peptides:
      gi|253570147    Mass: 54948    Score: 17     Queries matched: 1
 conserved hypothetical protein [Bacteroides sp. 1_1_6]

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 200 ppm
Fragment Mass Tolerance: ± 0.5 Da
Max Missed Cleavages   : 1
Instrument type        : Default
Number of queries      : 1

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 50 indicates identity
Score    Expect      ppm   Hit  Protein       Peptide
40.1 0.51 11.9 2 gi|493911 R.IVYSSDWLIYK.T
40.1 0.51 11.9 1 gi|133245 R.LVYSSDWLIYK.T
36.5 1.2 11.9 3 gi|493905 R.VLYSSDWLIYK.T
25.0 17 35.8 4 gi|229541855 -.MGYALEIEGLYK.S
21.6 37 -63.95 5 gi|229876264 R.IPVLIVSGFLGSGK.T
19.8 55 -66.82 6 gi|53712057 K.KLSFLVYIYIK.G
17.4 95 -3.29 7 gi|223038984 K.TLSKYEELLYK.D
17.1 1e+02 8.54 8 gi|168335189 K.IPHMGWNKLYK.T
17.1 1e+02 9.50 9 gi|224373298 K.KMDEFVTLIYK.Y
16.8 1.1e+02 -16.75 10 gi|29349483 R.YMKIIVDTIYK.M