Mascot Search Results

User            : 
Email           : jpuddick@waikato.ac.nz
Search title    : 
MS data file    : DATA.TXT
Database        : NCBInr 20090704 (9251875 sequences; 3169794832 residues)
Taxonomy        : Bacteria (Eubacteria) (4922962 sequences)
Timestamp       : 10 Jul 2009 at 02:08:16 GMT
Protein hits    : gi|133245 RecName: Full=Ribonuclease; AltName: Full=Binase; AltName: Full=RNase Bi; Flags: Precursor
  gi|493911 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
  gi|493905 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
  gi|229541855 ABC transporter related [Bacillus coagulans 36D1]
  gi|23097960 hypothetical protein OB0505 [Oceanobacillus iheyensis HTE831]
  gi|229876264 predicted GTPase, G3E family [Sphaerobacter thermophilus DSM 20745]
  gi|153953866 hypothetical protein CKL_1241 [Clostridium kluyveri DSM 555]
  gi|91215345 putative exoribonuclease [Psychroflexus torquis ATCC 700755]
  gi|33861335 putative cobalamin synthesis protein [Prochlorococcus marinus subsp. pastoris str. CCMP1986]
  gi|229163460 DNA recombinase [Bacillus cereus R309803]

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 46 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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Overview Table

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Hit: 1 2 3 4 5 6 7 8 9 10
1386.7830 (1+)

        Error tolerant   

1.    gi|133245    Mass: 17945    Score: 38     Queries matched: 1
 RecName: Full=Ribonuclease; AltName: Full=Binase; AltName: Full=RNase Bi; Flags: Precursor
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.7231   38.0 0  38  0.31 1   R.LVYSSDWLIYK.T

 
      Proteins matching the same set of peptides:
      gi|585908    Mass: 12174    Score: 38     Queries matched: 1
 RecName: Full=Ribonuclease; AltName: Full=RNase Bco
      gi|1350819    Mass: 17903    Score: 38     Queries matched: 1
 RecName: Full=Ribonuclease; AltName: Full=RNase Bp; Flags: Precursor
      gi|1942120    Mass: 12181    Score: 38     Queries matched: 1
 Chain A, Structure Of Binase Mutant His 101 Asn
      gi|17942905    Mass: 12950    Score: 38     Queries matched: 1
 Chain A, Hydrolase(Endoribonuclease)ribonuclease Bi (G Specific Endonuclease) (E.C.3.1.27.-) Complexed With Guanosine-3'- Phosphate (3'-Gmp)
      gi|157693862    Mass: 17900    Score: 38     Queries matched: 1
 guanine-specific ribonuclease [Bacillus pumilus SAFR-032]
      gi|157838324    Mass: 12159    Score: 38     Queries matched: 1
 Chain A, Structure Of Binase In Solution
      gi|194015154    Mass: 17899    Score: 38     Queries matched: 1
 ribonuclease (RNase Bp) [Bacillus pumilus ATCC 7061]


2.    gi|493911    Mass: 12361    Score: 38     Queries matched: 1
 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.7231   38.0 0  38  0.31 1   R.IVYSSDWLIYK.T


3.    gi|493905    Mass: 12361    Score: 34     Queries matched: 1
 Chain A, Crystal Structural Analysis Of Mutations In The Hydrophobic Cores Of Barnase
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.7231   38.0 0  34  0.8 3   R.VLYSSDWLIYK.T


4.    gi|229541855    Mass: 32661    Score: 25     Queries matched: 1
 ABC transporter related [Bacillus coagulans 36D1]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.6900   61.8 0  25  7.2 4   -.MGYALEIEGLYK.S


5.    gi|23097960    Mass: 42924    Score: 20     Queries matched: 1
 hypothetical protein OB0505 [Oceanobacillus iheyensis HTE831]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.8170   -29.76 0  20  22 5   K.VLPLLYIALEDK.A


6.    gi|229876264    Mass: 36486    Score: 16     Queries matched: 1
 predicted GTPase, G3E family [Sphaerobacter thermophilus DSM 20745]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.8282   -37.90 0  16  51 6   R.IPVLIVSGFLGSGK.T


7.    gi|153953866    Mass: 33860    Score: 13     Queries matched: 1
 hypothetical protein CKL_1241 [Clostridium kluyveri DSM 555]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.8282   -37.89 0  13  96 7   R.LVPIGSVAVQYLK.N

 
      Proteins matching the same set of peptides:
      gi|219854481    Mass: 34199    Score: 13     Queries matched: 1
 hypothetical protein CKR_1138 [Clostridium kluyveri NBRC 12016]

8.    gi|91215345    Score: 13     Queries matched: 1
 putative exoribonuclease [Psychroflexus torquis ATCC 700755]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.6649   80.0 0  13  1e+02 8   K.MYQLGEEVYVR.V


9.    gi|33861335    Mass: 39864    Score: 11     Queries matched: 1
 putative cobalamin synthesis protein [Prochlorococcus marinus subsp. pastoris str. CCMP1986]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.8282   -37.90 0  11  1.5e+02 9   R.IPVIVISGFLGSGK.T


10.   gi|229163460    Score: 11     Queries matched: 1
 DNA recombinase [Bacillus cereus R309803]
Check to include this hit in error tolerant search
       
      Query  Observed  Mr(expt)  Mr(calc)   ppm  Miss Score Expect Rank  Peptide
 1   1386.7830   1385.7757   1385.7190   40.9 0  11  1.7e+02 10   R.NDEIEHLILYK.R

 
      Proteins matching the same set of peptides:
      gi|229175176    Score: 11     Queries matched: 1

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 200 ppm
Fragment Mass Tolerance: ± 0.5 Da
Max Missed Cleavages   : 0
Instrument type        : Default
Number of queries      : 1

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 46 indicates identity
Score    Expect      ppm   Hit  Protein       Peptide
38.3 0.31 38.0 2 gi|493911 R.IVYSSDWLIYK.T
38.3 0.31 38.0 1 gi|133245 R.LVYSSDWLIYK.T
34.1 0.8 38.0 3 gi|493905 R.VLYSSDWLIYK.T
24.6 7.2 61.8 4 gi|229541855 -.MGYALEIEGLYK.S
19.7 22 -29.76 5 gi|23097960 K.VLPLLYIALEDK.A
16.1 51 -37.90 6 gi|229876264 R.IPVLIVSGFLGSGK.T
13.4 96 -37.89 7 gi|153953866 R.LVPIGSVAVQYLK.N
13.2 1e+02 80.0 8 gi|91215345 K.MYQLGEEVYVR.V
11.4 1.5e+02 -37.90 9 gi|33861335 R.IPVIVISGFLGSGK.T
10.9 1.7e+02 40.9 10 gi|229163460 R.NDEIEHLILYK.R