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dc.contributor.advisorBird, Steve
dc.contributor.authorGibbons, Olivia Robyn
dc.date.accessioned2017-09-20T03:54:21Z
dc.date.available2017-09-20T03:54:21Z
dc.date.issued2016
dc.identifier.citationGibbons, O. R. (2016). Antimicrobial Peptides in Jawed and Jawless Vertebrates (Thesis, Master of Science (Research) (MSc(Research))). University of Waikato, Hamilton, New Zealand. Retrieved from http://hdl.handle.net/10289/11349en
dc.identifier.urihttp://hdl.handle.net/10289/11349
dc.description.abstractAntimicrobial peptides (AMPs) are a major part of the innate immune defence system which shows a broad spectrum of activity, defending the host against invading microbes. The aim of this work was to identify the AMPs present in yellowtail kingfish (Seriola lalandi) and pouched lamprey (Geotria australis) and use molecular techniques to fully sequence their cDNA and quantify their expression in adult individuals. Using bioinformatic approaches candidate AMP genes were ascertained from available S. lalandi and G. australis RNA-seq transcriptomic databases, obtained from various tissues. Selected AMPs were chosen to have their full cDNA sequence amplified using RACE-PCR, which were then cloned and sequenced. Complete cDNA sequences were obtained for S. lalandi hepcidin and moronecidin, whereas attempts to complete the G. australis defensin-like cDNA were unsuccessful. Comparison of the S. lalandi hepcidin and moronecidin protein sequences with proteins already characterised in other fish showed good homology and conservation of important features. In addition, specific primers were designed to examine the expression levels of S. lalandi hepcidin and moronecidin in gill, liver or spleens of three fish. Analysis showed hepcidin expression to be highest in liver tissues, whereas moronecidin expression was highest in the gills and spleens. This study provides a comprehensive overview of the AMP genes present in S. lalandi and G. australis and some initial characterisation of S. lalandi hepcidin and moronecidin, which will permit the development of future research applications. Overall, characterising AMP genes in jawed and jawless vertebrates is vital for economical and successful fish farming, while also providing possible therapeutic benefits associated with AMP research in biomedicine and disease in wild fish stocks.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherUniversity of Waikato
dc.rightsAll items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectAntimicrobial Peptides
dc.subjectVertebrates
dc.subjectMoronecidin
dc.subjectHepcidin
dc.subjectDefensin-like
dc.subjectLamprey
dc.subjectYellowtail Kingfish
dc.titleAntimicrobial Peptides in Jawed and Jawless Vertebrates
dc.typeThesis
thesis.degree.grantorUniversity of Waikato
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (Research) (MSc(Research))
dc.date.updated2016-02-25T21:57:57Z
pubs.place-of-publicationHamilton, New Zealand


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