Balog, E., Becker, T., Oettl, M., Lechner, R., Daniel, R. M., Finney, J. & Smith, J. C. (2004). Direct determination of vibrational density of states change on ligand binding to a protein. Physical Review letters, 93(2), 028103.
Permanent Research Commons link: http://hdl.handle.net/10289/3796
The change in the vibrational density of states of a protein (dihydrofolate reductase) on binding a ligand (methotrexate) is determined using inelastic neutron scattering. The vibrations of the complex soften significantly relative to the unbound protein. The resulting free-energy change, which is directly determined by the density of states change, is found to contribute significantly to the binding equilibrium.
American Physical Society
This article has been published in the journal: Physical Review letters. ©2004 The American Physical Society.