Uhl, A.M. & Daniel, R.M. (1999). The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1. Extremophiles, 3(4), 263-267.
Permanent Research Commons link: http://hdl.handle.net/10289/4463
A xylanase has been found in the archaeon Thermococcus zilligii strain AN1 (DSM 2770), which grows optimally at 75°C. The enzyme had a molecular mass of 95 kDa and a unique N-terminal sequence. It had activity against all five xylans tested and against xylose oligomers, but not against other carbohydrate polymers. The K m values found for xylans were typical of those found for bacterial xylanases. The pH optimum for activity was pH 6, and the enzyme half-life at 100°C was 8 min. This is the first description of any archaeal hemicellulase.