A general method for the synthesis of peptidyl substrate for proteolytic enzymes

A general method for the synthesis of peptidyl substrate for proteolytic enzymes

Whitmore, Andrew ; Daniel, Roy M. ; Petach, Helen H.

Identifier: http://hdl.handle.net/10289/4469

Date: 1995

Citation: Whitmore, A., Daniel, R.M. & Petach, H.H. (1995). A general method for the synthesis of peptidyl substrate for proteolytic enzymes. Tetrahedron Letters, 36(3), 475-476.

Publisher: Elsevier

Abstract:

The synthesis of an heterogeneous peptide substrate for the assay of proteases was carried out by cleaving a protein using a protease to generate small peptides which were then coupled to a chromophore, p-nitroaniline. The chromophoric peptide product could be used to assay for the protease which produced the original peptides.

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