Health, C., Hu, X.P., Cary, S.C. & Cowan, D. (2009). Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome. Applied and Environmental Microbiology, 77, 1153-1161.
Permanent Research Commons link: http://hdl.handle.net/10289/5382
A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application.
American Society for Microbiology