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Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome

Abstract
A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application.
Type
Journal Article
Type of thesis
Series
Citation
Health, C., Hu, X.P., Cary, S.C. & Cowan, D. (2009). Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome. Applied and Environmental Microbiology, 77, 1153-1161.
Date
2009
Publisher
American Society for Microbiology
Degree
Supervisors
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