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Development of an HPLC method to analyze four veterinary antibiotics in soils and aqueous media and validation through fate studies

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dc.contributor.author Srinivasan, Prakash
dc.contributor.author Sarmah, Ajit K.
dc.contributor.author Manley-Harris, Merilyn
dc.contributor.author Wilkins, Alistair L.
dc.date.accessioned 2012-10-02T03:36:30Z
dc.date.available 2012-10-02T03:36:30Z
dc.date.issued 2012
dc.identifier.citation Srinivasan, P., Sarmah, A., Manley-Harris, M. & Wilkins, A. (2012). Development of an HPLC method to analyze four veterinary antibiotics in soils and aqueous media and validation through fate studies. Journal of Environmental Science and Health Part A-Toxic/Hazardous Substances & Environmental Engineering, 47(13), 2120-2132. en_NZ
dc.identifier.issn 1093-4529
dc.identifier.uri http://hdl.handle.net/10289/6672
dc.description.abstract A simple, yet robust analytical method was developed to detect and quantify three sulfonamides (SA), namely sulfamethoxazole (SMO), sulfachloropyridazine (SCP), and sulfamethazine (SM), and a macrolide tylosin (TT) in aqueous (calcium chloride and leachate solutions) and solid (agricultural soils) matrices using high performance liquid chromatography and ultra violet detection at 290 nm (TT) and 275 nm (SA) respectively. Chromatography was performed using a Phenomenex Onyx Monolithic C-18 column for TT and a C-18 Luna column for sulfonamides as single analytes eluted isocratically with a mobile phase consisting of acetonitrile: trifluoroacetic acid: tetrahydrofuran in the ratio 22.5:68:9.5 for TT, 40:55:5 for SMO, 32:63:5 for SCP and 31:64:5 for SM (v/v) at 1.0 mL min(-1) and an injection volume of 20 mu L. A gradient method to detect all three sulfonamides in a single run was also developed. The soil residue analysis consisted of extraction with dichloromethane and pre-concentration steps as the aqueous phase was measured directly. The limits of detection at an S/N (signal: noise) ratio of 3 were 20.0 mu g L-1 and 50 mu g L-1 for all sulfonamides and tylosin respectively. The average recoveries for all sulfonamides and tylosin in aqueous matrices ranged from 95 to 105% across the six concentrations investigated. Recoveries from the soils were slightly lower for sulfonamides and tylosin. The isocratic method was used to determine the sorption and degradation of sulfonamides in soils, while the gradient method was used to determine degradation kinetics and leachate concentrations in soils and aqueous systems. en_NZ
dc.language.iso en
dc.publisher Taylor & Francis en_NZ
dc.subject HPLC en_NZ
dc.subject Veterinary antibiotics en_NZ
dc.subject Soil en_NZ
dc.subject sulfonamides en_NZ
dc.subject Macrolide en_NZ
dc.subject Sorption en_NZ
dc.subject Recovery en_NZ
dc.title Development of an HPLC method to analyze four veterinary antibiotics in soils and aqueous media and validation through fate studies en_NZ
dc.type Journal Article en_NZ
dc.identifier.doi 10.1080/10934529.2012.696005 en_NZ


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