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dc.contributor.authorSmith, Kirsty Fiona
dc.contributor.authorThia, Joshua
dc.contributor.authorGemmill, Chrissen E.C.
dc.contributor.authorCary, S. Craig
dc.contributor.authorFidler, Andrew E.
dc.date.accessioned2012-10-04T03:00:27Z
dc.date.available2012-10-04T03:00:27Z
dc.date.copyright2012
dc.date.issued2012
dc.identifier.citationSmith, K.F., Thia, J., Gemmill, C.E.C., Cary, S.C., & Fidler, A.e. (2012). Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination. Aquatic Invasions, 7(3), 305-313.en_NZ
dc.identifier.issn1798-6540
dc.identifier.urihttp://hdl.handle.net/10289/6680
dc.description.abstractMitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C. savignyi COI genetic diversity around the NZ archipelago and to compare this with diversity within C. savignyi's native range in the north-west Pacific. Ciona savignyi specimens were collected from two NZ sites and from three sites around Japan. COI sequences (595 bp) were amplified and measures of genetic diversity were calculated. Based on differences between their COI sequences we developed a PCR-based assay to distinguish C. savignyi from the morphologically similar C. intestinalis. A total of 12 C. savignyi COI haplotypes were recovered from the 76 samples. Of the four haplotypes observed in NZ, two were unique. From the 10 haplotypes observed in the Japan samples, eight were unique. The C. savignyi populations in Japan were found to contain higher haplotype diversity when compared with those in NZ. The NZ samples contained only a small subset of the haplotype variation of the Japan samples, however, NZ samples did harbor two haplotypes not observed in the Japan samples. A PCR-based assay developed from the COI sequences was able to reliably discriminate the two Ciona species. The low COI genetic diversity within the two NZ C. savignyi populations sampled is consistent with a founder effect associated loss of genetic diversity. The robust PCR-based assay for distinguishing C. savignyi and C. intestinalis may find application in ecological and taxonomic studies and can be applied to both archival materials and live animals.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherRegional Euro-Asian Biological Invasions Centre (REABIC)en_NZ
dc.relation.ispartofAquatic Invasions
dc.rights© 2012 The authorsen_NZ
dc.subjectCryptic invasionen_NZ
dc.subjectMitochondrial cytochrome oxidase I (COI)en_NZ
dc.subjectSpecies identificationen_NZ
dc.subjectDNA barcodingen_NZ
dc.subjectBiosecurityen_NZ
dc.subjectInvasive speciesen_NZ
dc.titleBarcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discriminationen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.3391/ai.2012.7.3.002en_NZ
pubs.elements-id37295


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