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Survey of Scytonema (Cyanobacteria) and associated saxitoxins in the littoral zone of recreational lakes in Canterbury, New Zealand

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dc.contributor.author Smith, Francine M. J.
dc.contributor.author Wood, Susanna A.
dc.contributor.author Wilks, Taryn
dc.contributor.author Kelly, David
dc.contributor.author Broady, Paul A.
dc.contributor.author Williamson, Wendy M.
dc.contributor.author Gaw, Sally K.
dc.date.accessioned 2012-10-11T23:23:31Z
dc.date.available 2012-10-11T23:23:31Z
dc.date.copyright 2012-09-04
dc.date.issued 2012
dc.identifier.citation Smith, F. M. J., Wood, S. A., Wilks, T., Kelly, D., Broady, P. A., Williamson, W.M., & Gaw, S.K. (2012). Survey of Scytonema (Cyanobacteria) and associated saxitoxins in the littoral zone of recreational lakes in Canterbury, New Zealand. Phycologia, 51(5), 542-551. en_NZ
dc.identifier.issn 0031-8884
dc.identifier.uri http://hdl.handle.net/10289/6719
dc.description.abstract The recent identification of saxitoxin-producing Scytonema cf. crispum triggered a survey of metaphyton and periphyton for Scytonema spp. in 34 high-use recreational lakes across Canterbury, New Zealand. Scytonema was observed in 10 of the lakes surveyed. Three morphospecies were identified: Scytonema cf. crispum, Scytonema cf. chiastum and Scytonema cf.fritschii. Environmental samples containing Scytonema were analysed for saxitoxins using the Jellett rapid test for paralytic shellfish poisoning, and saxitoxin variants were identified in positive samples using high-performance liquid chromatography with fluorescence detection (HPLC-FD). Cultures were established from selected sites and their phylogeny investigated using partial 16S rRNA gene sequences. These cultures were also screened for a region of sxtA, a gene involved in saxitoxin production. Cultures containing the sxtA gene were analysed for saxitoxins with HPLC-FD. Saxitoxins were only identified in cultures of S. cf. crispum and environmental samples containing this species. HPLC-FD analysis of these environmental samples and cultures identified saxitoxin and the variants gonyautoxins (GTX1-5), neosaxitoxin, decarbamoyl saxitoxin and decarbamoyl gonyautoxins (dcGTX2/3). This was the first report of these saxitoxin variants from cyanobacteria in New Zealand. All cultures of S. cf. crispum contained the sxtA gene segment. The partial 16S rRNA gene sequence of Scytonema C. Agardh ex Bornet & Flahault cultures were compared with cyanobacterial sequences from GenBank, only S. cf.fritschii clustered amongst other Scytonema species. The identification of metaphytic saxitoxin-producing S. cf. crispum highlighted a new freshwater habitat where toxic cyanobacteria may need to be monitored. en_NZ
dc.language.iso en
dc.publisher International Phycological Society en_NZ
dc.relation.ispartof Phycologia
dc.subject 16S rRNA en_NZ
dc.subject Blue-green algae en_NZ
dc.subject Metaphyton en_NZ
dc.subject Paralytic shellfish poisoning toxins en_NZ
dc.subject PSP en_NZ
dc.subject SxtA gene en_NZ
dc.title Survey of Scytonema (Cyanobacteria) and associated saxitoxins in the littoral zone of recreational lakes in Canterbury, New Zealand en_NZ
dc.type Journal Article en_NZ
dc.identifier.doi 10.2216/11-84.1 en_NZ


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