Gill,B.D., Indyk, H.E., Kumar, M.C., Sievwright, N.K., Manley-Harris, M. & Dowell, D. (2012). Analysis of 5'-mononucleotides in infant formula and adult/pediatric nutritional formula by liquid chromatography: First action 2011.20. Journal of AOAC International, 95(3), 599-602.
Permanent Research Commons link: http://hdl.handle.net/10289/6738
A method for the routine determination of 5'-mononucleotides (uridine 5'-monophosphate, inosine 5'-monophosphate, adenosine 5'-monophosphate, guanosine 5'-monophosphate, and cytidine 5'-monophosphate) in infant formula and adult nutritionals is described. After sample dissolution and addition of internal standard, potential interferences were removed by anion-exchange SPE followed by HPLC-UV analysis. Single-laboratory validation performance parameters include recovery (92-101%) and repeatability (1.0-2.3% RSD). The method was approved for Official First Action status by an AOAC expert review panel