Enzyme activity down to −100°C

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dc.contributor.authorBragger, J.M.
dc.contributor.authorDunn, Rachel V.
dc.contributor.authorDaniel, Roy M.
dc.date.accessioned2010-08-27T04:27:38Z
dc.date.available2010-08-27T04:27:38Z
dc.date.issued2000
dc.description.abstractThe activities of two enzymes, beef liver catalase (EC 1.11.1.6) and calf intestine alkaline phosphatase (EC 3.1.3.1), have been measured down to −97°C and −100°C, respectively. Enzyme activity has not previously been measured at such low temperatures. For catalase, the cryosolvents used were methanol:ethylene glycol:water (70:10:20) and DMSO:ethylene glycol:water (60:20:20). For alkaline phosphatase, methanol:ethylene glycol:water (70:10:20) was used. All of the Arrhenius plots were linear over the whole of the temperature range examined. Since the lowest temperatures at which activity was measured are well below the dynamic transition observed for proteins, the results indicate that the motions which cease below the dynamic transition are not essential for enzyme activity. In all cases the use of cryosolvent led to substantial increases in Arrhenius activation energies, and this imposed practical limitations on the measurement of enzyme activity below −100°C. At even lower temperatures, enzyme activity may be limited by the effect of solvent fluidity on substrate/product diffusion, but overall there is no evidence that any intrinsic enzyme property imposes a lower temperature limit for enzyme activity.en_NZ
dc.identifier.citationBragger, J.M., Dunn, R.V. & Daniel, R.M. (2000). Enzyme activity down to −100°C. Biochimica et Biophysica Acta (BBA) – Protein Structure and Molecular Enzymology, 1480(1-2), 278-282.en_NZ
dc.identifier.doi10.1016/S0167-4838(00)00081-9en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4449
dc.language.isoen
dc.publisherElsevieren_NZ
dc.relation.isPartOfBiochimica et Biophysica Actaen_NZ
dc.subjectAlkaline phosphatiseen_NZ
dc.subjectcatalase
dc.subjectcryoenzymology
dc.subjectcryosolvent
dc.subjectexobiology
dc.subjectprotein dynamics
dc.titleEnzyme activity down to −100°Cen_NZ
dc.typeJournal Articleen_NZ
pubs.begin-page278en_NZ
pubs.elements-id41394
pubs.end-page282en_NZ
pubs.volume1480en_NZ
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