Molecular genetic tools for environmental monitoring of New Zealand's aquatic habitats, past, present and the future

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dc.contributor.authorWood, Susanna A.
dc.contributor.authorSmith, Kirsty Fiona
dc.contributor.authorBanks, Jonathan C.
dc.contributor.authorTremblay, Louis A.
dc.contributor.authorRhodes, Lesley L.
dc.contributor.authorMountfort, Douglas O.
dc.contributor.authorCary, S. Craig
dc.contributor.authorPochon, Xavier
dc.date.accessioned2013-03-17T20:14:10Z
dc.date.available2013-03-17T20:14:10Z
dc.date.copyright2013-03
dc.date.issued2013
dc.description.abstractThe assessment of biological samples is critical for measuring the health of New Zealand aquatic environments. Analysis of these samples commonly requires species identification and enumeration, which usually involves microscopy or microbiological methods. These techniques can be time-consuming, laborious, and are dependent on taxonomic expertise. Recent advances in molecular methods provide promising tools for assessing environmental samples. A range of molecular techniques are now used in New Zealand including: fluorescent in situ hybridisation; automated ribosomal intergenic spacer analysis; quantitative polymerase chain reaction; and, most recently, next-generation sequencing. The organisms (or targets) and environments monitored are equally diverse, ranging from cyanobacteria, rotifers and invasive fish in lakes, to macroinvertebrates, and biofilm communities in rivers, to bacteria, micro-and macro-algae and invertebrates in marine ecosystems. Despite research and validation demonstrating their potential, the application of these tools by monitoring agencies has been limited. Legislative requirements, costs, and a reluctance to change methodologies, are the most likely reasons for this. This review examines molecular tools that have been previously or are currently used for monitoring aquatic environments in New Zealand, and explores how these, and new techniques, may be applied in the future.en_NZ
dc.identifier.citationWood, S., Smith, K., Banks, J., Tremblay, L., Rhodes, L., Mountfort, D., & Pochon, X. (2013). Molecular genetic tools for environmental monitoring of New Zealand s aquatic habitats, past, present and the future. New Zealand Journal of Marine and Freshwater Research, 47(1), 90-119.en_NZ
dc.identifier.doi10.1080/00288330.2012.745885en_NZ
dc.identifier.issn0028-8330
dc.identifier.urihttps://hdl.handle.net/10289/7368
dc.language.isoen
dc.publisherThe Royal Society of New Zealand.en_NZ
dc.relation.isPartOfNew Zealand Journal of Marine and Freshwater Researchen_NZ
dc.relation.ispartofNew Zealand Journal of Marine and Freshwater Research
dc.subjectautomated ribosomal intergenic spacer analysisen_NZ
dc.subjectfluorescent in situ hybridisationen_NZ
dc.subjectnext-generation sequencingen_NZ
dc.subjectpolymerase chain reactionen_NZ
dc.subjectquantitative polymerase chain reactionen_NZ
dc.subjectsandwich hybridisation arraysen_NZ
dc.subjectSanger DNA sequencingen_NZ
dc.subjectterminal restriction fragment length polymorphismen_NZ
dc.titleMolecular genetic tools for environmental monitoring of New Zealand's aquatic habitats, past, present and the futureen_NZ
dc.typeJournal Articleen_NZ
pubs.begin-page90en_NZ
pubs.elements-id38352
pubs.end-page119en_NZ
pubs.issue1en_NZ
pubs.volume47en_NZ
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