First demonstration of antigen induced cytokine expression by CD4-1⁺ lymphocytes in a poikilotherm: Studies in Zebrafish (Danio rerio)

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dc.contributor.authorYoon, Sohyeen_NZ
dc.contributor.authorMitra, Sumanen_NZ
dc.contributor.authorWyse, Cathyen_NZ
dc.contributor.authorAlnabulsi, Ayhamen_NZ
dc.contributor.authorZou, Junen_NZ
dc.contributor.authorWeerdenburg, Eveline M.en_NZ
dc.contributor.authorvan der Sar, Astrid M.en_NZ
dc.contributor.authorWang, Difeien_NZ
dc.contributor.authorSecombes, Christopher J.en_NZ
dc.contributor.authorBird, Steveen_NZ
dc.contributor.editorFischer, Uween_NZ
dc.date.accessioned2015-07-08T03:00:30Z
dc.date.available2015-06-17en_NZ
dc.date.available2015-07-08T03:00:30Z
dc.date.issued2015-06-17en_NZ
dc.description.abstractAdaptive immunity in homeotherms depends greatly on CD4+ Th cells which release cytokines in response to specific antigen stimulation. Whilst bony fish and poikilothermic tetrapods possess cells that express TcR and CD4-related genes (that exist in two forms in teleost fish; termed CD4-1 and CD4-2), to date there is no unequivocal demonstration that cells equivalent to Th exist. Thus, in this study we determined whether CD4-1⁺ lymphocytes can express cytokines typical of Th cells following antigen specific stimulation, using the zebrafish (Danio rerio). Initially, we analyzed the CD4 locus in zebrafish and found three CD4 homologues, a CD4-1 molecule and two CD4-2 molecules. The zfCD4-1 and zfCD4-2 transcripts were detected in immune organs and were most highly expressed in lymphocytes. A polyclonal antibody to zfCD4-1 was developed and used with an antibody to ZAP70 and revealed double positive cells by immunohistochemistry, and in the Mycobacterium marinum disease model CD4-1⁺ cells were apparent surrounding the granulomas typical of the infection. Next a prime-boost experiment, using human gamma globulin as antigen, was performed and revealed for the first time in fish that zfCD4-1⁺ lymphocytes increase the expression of cytokines and master transcription factors relevant to Th1/Th2- type responses as a consequence of boosting with specific antigen.
dc.format.mimetypeapplication/pdf
dc.identifier.citationYoon, S., Mitra, S., Wyse, C., Alnabulsi, A., Zou, J., Weerdenburg, E. M., … Bird, S. (2015). First demonstration of antigen induced cytokine expression by CD4-1⁺ lymphocytes in a poikilotherm: Studies in Zebrafish (Danio rerio). PLOS ONE, 10(6), e0126378–e0126378. http://doi.org/10.1371/journal.pone.0126378en
dc.identifier.doi10.1371/journal.pone.0126378en_NZ
dc.identifier.eissn1932-6203en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/9450
dc.language.isoen
dc.publisherPublic Library of Scienceen_NZ
dc.relation.isPartOfPLOS ONEen_NZ
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126378en_NZ
dc.rights© 2015 Yoon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.titleFirst demonstration of antigen induced cytokine expression by CD4-1⁺ lymphocytes in a poikilotherm: Studies in Zebrafish (Danio rerio)en_NZ
dc.typeJournal Article
pubs.begin-pagee0126378
pubs.elements-id128098
pubs.end-pagee0126378
pubs.issue6en_NZ
pubs.notesEvidence of peer review: http://journals.plos.org/plosone/s/journal-informationen_NZ
pubs.organisational-group/Waikato
pubs.organisational-group/Waikato/FSEN
pubs.organisational-group/Waikato/FSEN/School of Science
pubs.volume10en_NZ
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