Role of Cx36 gap junction modulation in general anaesthetic anticonvulsant action

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dc.contributor.authorVoss, Logan J.
dc.contributor.authorMelin, Sofia M.
dc.contributor.authorJacobson, Gregory M.
dc.contributor.authorSleigh, James W.
dc.date.accessioned2010-08-02T01:22:28Z
dc.date.available2010-08-02T01:22:28Z
dc.date.issued2010
dc.description.abstractMany GABAergic anaesthetics reduce gap junction coupling but it is currently unknown whether this effect contributes to anaesthetic anticonvulsant action. In this study we examined the possible role of connexin36 gap junctions in the anticonvulsant action of isoflurane and compared this to etomidate, an anaesthetic known for having proconvulsant effects. We compared the effect of anaesthetic concentrations of isoflurane (1 MAC) and etomidate (16 μM) on low-magnesium-induced interictal-like activity in isolated neocortical slices. The effect of connexin36 gap junction blockade was explored by comparing effects in slices from wild-type mice and from a transgenic mouse strain lacking the gene for connexin36. In slices from wild-type mice, both isoflurane (1 MAC) and etomidate (16 μM) reduced interictal-like event frequency; mean(S.D.) reduction of 44(13)% (P < 0.0001) and 25(24)% (P < 0.0001), respectively. The reduction in event frequency was greater for isoflurane (P < 0.005). Isoflurane had no effect on the amplitude of interictal-like events, but event amplitude was enhanced by etomidate (18(28)% increase, P < 0.005). The capacity for isoflurane to reduce event frequency was significantly reduced, but not eliminated in slices from connexin36 knock-out mice (33(15)% reduction, P < 0.05 for the difference with wild-type), while that of etomidate remained unchanged (23(39)% reduction). The etomidate-mediated increase in event amplitude was eliminated in connexin36 knock-out slices. The results from this study support the hypothesis that the anticonvulsant effect of isoflurane is in part mediated by gap junction blockade. The role of gap junction modulation by etomidate is more complicated and may be important in the mechanism of action of etomidate's proconvulsant effects.en_NZ
dc.identifier.citationVoss, L.J., Melin, S., Jacobson, G. & Sleigh, J.W. (2010). Role of Cx36 gap junction modulation in general anaesthetic anticonvulsant action. European Journal of Pharmacology, 643(1), 58-62.en_NZ
dc.identifier.doi10.1016/j.ejphar.2010.06.038en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4226
dc.language.isoen
dc.publisherElsevieren_NZ
dc.relation.isPartOfEuropean Journal of Pharmacologyen_NZ
dc.subjectetomidateen_NZ
dc.subjectisofluraneen_NZ
dc.subjectgap junctionen_NZ
dc.subjectepileptiformen_NZ
dc.titleRole of Cx36 gap junction modulation in general anaesthetic anticonvulsant actionen_NZ
dc.typeJournal Articleen_NZ
pubs.begin-page58en_NZ
pubs.elements-id35201
pubs.end-page62en_NZ
pubs.issue1en_NZ
pubs.volume643en_NZ
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