Browsing by Author "Dunn, Rachel V."

Now showing items 6-10 of 13

  • Enzyme Dynamics and Activity: Time-Scale Dependence of Dynamical Transitions in Glutamate Dehydrogenase Solution

    Daniel, Roy M.; Finney, John L.; Reat, Valerie; Dunn, Rachel V.; Smith, Jeremy C. (1999-10)
    We have examined the temperature dependence of motions in a cryosolution of the enzyme glutamate dehydrogenase (GDH) and compared these with activity. Dynamic neutron scattering was performed with two instruments of different ...
  • Esterase catalysis of substrate vapour: enzyme activity occurs at very low hydration

    Lind, Penelope A.; Daniel, Roy M.; Monk, Colin R.; Dunn, Rachel V. (Elsevier, 2004-10)
    It has been generally accepted that enzyme activity requires a minimal hydration of about 0.2 g H2O g⁻¹ protein. This fits well with evidence that hydration above this level is associated with the onset of intramolecular ...
  • Low frequency enzyme dynamics as a function of temperature and hydration: A neutron scattering study

    Kurkal, Vandana; Daniel, Roy M.; Finney, John L.; Tehei, M.; Dunn, Rachel V.; Smith, Jeremy C. (Elsevier, 2005)
    The effect of hydration and temperature on the low-frequency dynamics of the enzyme Pig liver esterase has been investigated with incoherent neutron scattering experiments. The results suggest that at low temperature, ...
  • The role of dynamics in enzyme activity

    Daniel, Roy M.; Dunn, Rachel V.; Finney, John L.; Smith, Jeremy C. (Annual Reviews, 2003)
    Although protein function is thought to depend on flexibility, precisely how the dynamics of the molecule and its environment contribute to catalytic mechanisms is unclear. We review experimental and computational work ...
  • Solvent dependence of dynamic transitions in protein solutions

    Reat, Valerie; Dunn, Rachel V.; Ferrand, Michel; Finney, John L.; Daniel, Roy M.; Smith, Jeremy C. (2000)
    A transition as a function of increasing temperature from harmonic to anharmonic dynamics has been observed in globular proteins by using spectroscopic, scattering, and computer simulation techniques. We present here results ...