Browsing by Supervisor "Arcus, Vickery L."

Now showing items 1-20 of 27

  • Measuring the temperature response of soil respiration from two distinct carbon pools in soil.

    Robinson, Jasmine May (The University of Waikato, 2020)
    As soils contain a large store of terrestrial carbon, understanding the dynamics and stability of this important carbon reserve and how it may change with regards to future changes in temperature is of global interest. It ...
  • Finding the good vibes of life, man: Identifying catalytic vibrations in enzyme catalysis

    Schipper, Daniel-Albert (The University of Waikato, 2020)
    Enzyme catalysis is one of the most vital components of life. As such the elucidation of the exact mechanics of this kind of catalysis is important. The aim of this work is to look into enzyme catalysis though the lens of ...
  • Understanding allosteric enzyme regulation using macromolecular rate theory

    Hamill, Carlin James (The University of Waikato, 2020)
    Enzyme catalysed reaction rates produce a curved temperature dependence with a temperature optimum, Topt. Traditionally enzyme temperature dependence has been modelled by the Arrhenius and Eyring-Polanyi equations. Deviations ...
  • Characterisation of the FitAB Toxin-Antitoxin System in Neisseria gonorrhoeae

    Warrender, Annmaree (The University of Waikato, 2019)
    Neisseria gonorrhoeae is the causative agent of the sexually transmitted infection, gonorrhoea. In a standard infection, N. gonorrhoeae invade epithelial cells lining the urogenital tract and exit into the sub-epithelial ...
  • The structure and function of a bovine salivary protein - BSP30b

    Zhang, Heng (The University of Waikato, 2019)
    The Bovine Salivary Protein 30b (BSP30b) is a member of the palate, lung, and nasal epithelium clone (PLUNC) family which includes human bactericidal/permeability-increasing protein (BPI) and lipopolysaccaride binding ...
  • Mycobacterium tuberculosis strains in New Zealand: phylogeny and structural biology

    Claire Vignette, Mulholland (The University of Waikato, 2019)
    Mycobacterium tuberculosis is an obligate human pathogen and is the primary causative agent of tuberculosis. New Zealand has a relatively low incidence of tuberculosis disease, however, Māori (the indigenous people of New ...
  • Do PhoH2 proteins regulate SigF in mycobacteria?

    Rzoska-Smith, Elizabeth (The University of Waikato, 2018)
    There is little known about the role of the PhoH2 proteins in biology. PhoH2 consists of two domains, an N-terminal PIN domain with RNase activity and a C-terminal PhoH domain shown to have ATP induced RNA helicase activity. ...
  • Evolution of Bacillus subtilis: A Novel Phenotype and its Challenges

    Murray, Mitchell John (The University of Waikato, 2018)
    The enzyme LeuB from the last common ancestor of the firmicutes has been statistically recreated using several different methods. This project deals with two versions, Rec/LG and Rec/EX. To better understand these resurrected ...
  • Engineering and characterisation of anti-progesterone OBodies

    Chonira, Vikas Kariyappa (The University of Waikato, 2018)
    Molecular interactions are fundamental to communication between different parts of the cell or of an organism. These interactions can be weak and transient or strong and semi-permanent. In the case of the adaptive immune ...
  • Investigating Macromolecular Rate Theory

    Prentice, Erica Jean (The University of Waikato, 2017)
    Curvature with temperature is a defining characteristic of enzyme catalysed rates. Historically, curvature has been described by Arrhenius behaviour up to a temperature optimum (Tₒₚₜ), while decreases in rates above Tₒₚₜ ...
  • Caldalkalibacillus thermarum PhoH2: Solving the solubility puzzle

    Dillon, Brooke Jane (University of Waikato, 2017)
    Previous attempts to determine the structure of the protein PhoH2 from Mycobacterium tuberculosis, Mycobacterium smegmatis and Thermobispora bispora have been unsuccessful producing diffraction data to low resolution. The ...
  • Testing Macromolecular Rate Theory

    Kraakman, Kirsty Leigh (University of Waikato, 2017)
    Enzymatic rate increase with respect to temperature has widely been described by transition-state theory. The experimentally observed rate decline above an optimum temperature (Tₒₚₜ) for enzymes has previously been attributed ...
  • Regulation of Metabolism in Mycobacteria and Cyanobacteria

    Vickers, Chelsea (University of Waikato, 2015)
    Bacteria encounter changes in their environment and must adapt to these changes in order to survive. Their ability to adapt is determined by their capacity to efficiently regulate their cellular processes. The mechanisms ...
  • The evolution of AroA and MurA enzymes from Bacillus

    Oulavallickal, Tifany (University of Waikato, 2016)
    MurA and AroA are important antibacterial targets due to their essentiality in microorganisms and the absence of their respective pathways within mammals. Although much research has focussed on these enzymes, little is ...
  • Time to Diagnosis and Persistence: The Two Major Determinants of Effective Tuberculosis Control

    Ruthe, Alaine Tess (University of Waikato, 2015)
    The greatest challenge confronting effective tuberculosis (TB) eradication is the time to diagnosis, and duration of treatment of chronically infected individuals which represent a pool of infection. In an attempt to help ...
  • Identifying the signalling pathway of a novel Myostatin Splice Variant (MSV)

    Hennebry, Alex (University of Waikato, 2014)
    Myostatin (Mstn), a member of the transforming growth factor-β super family, is a potent negative regulator of skeletal muscle mass. Studies delineating the function of Mstn have identified multiple signal transduction ...
  • Characterisation of Enzyme Evolution through Ancestral Enzyme Reconstruction

    Prentice, Erica Jean (University of Waikato, 2013)
    Through ancestral sequence reconstruction (ASR) techniques, ancient enzymes can be recreated and biochemically tested, giving insight into the enzymes’ evolutionary history. A previous study by Hobbs et al. (2012) has ...
  • Characterisation of VapBC Toxin-Antitoxins from Mycobacterium tuberculosis

    Sharrock, Abigail Victoria (University of Waikato, 2013)
    Toxin-antitoxin (TA) systems were identified more than 20 years ago on the mini F plasmid of Escherichia coli as plasmid stability elements; components responsible for purging bacterial cells that lack the plasmid from the ...
  • The Biology and Biochemistry of PhoH2 proteins

    Andrews, Emma Sophie Vout (University of Waikato, 2013)
    PhoH2 proteins are found in a diverse range of organisms. To date little is known about these proteins and the role they play in the organisms in which they reside. PhoH2 is a PIN PhoH domain fusion, and these proteins are ...
  • Biochemical Characterisation of Reconstructed Ancestral CM-DAH7PS Enzymes

    McMillan, Joel Patrick (University of Waikato, 2012)
    Thermophily has been proposed to be a primitive trait (Stetter, 2006, Pace, 1991, Woese, 1987) which has led to suggestions that all contemporary thermophilic species are the direct descendants of ancient thermophilic ...