Browsing by Supervisor "Arcus, Vickery L."
Now showing items 1-20 of 29
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Understanding enzymatic mechanism and allostery using macromolecular rate theory
(The University of Waikato, 2022)Intricate systems of regulation at many levels act to control enzyme rates to tune metabolism across biosynthetic pathways. Allostery is a ubiquitous regulatory mechanism for feedback and regulation of enzymes in biosynthetic ... -
Verifying clustered mutation regions inside the newly-completed genome of a clinically-significant New Zealand Mycobacterium tuberculosis strain
(The University of Waikato, 2021)In 2019, active infections of Mycobacterium tuberculosis (Mtb) affected 10 million people globally, with 1.4 million deaths, and an additional 1.7-1.9 billion people infected without symptomatic disease (latent Mtb). While ... -
Measuring the temperature response of soil respiration from two distinct carbon pools in soil.
(The University of Waikato, 2020)As soils contain a large store of terrestrial carbon, understanding the dynamics and stability of this important carbon reserve and how it may change with regards to future changes in temperature is of global interest. It ... -
Finding the good vibes of life, man: Identifying catalytic vibrations in enzyme catalysis
(The University of Waikato, 2020)Enzyme catalysis is one of the most vital components of life. As such the elucidation of the exact mechanics of this kind of catalysis is important. The aim of this work is to look into enzyme catalysis though the lens of ... -
Understanding allosteric enzyme regulation using macromolecular rate theory
(The University of Waikato, 2020)Enzyme catalysed reaction rates produce a curved temperature dependence with a temperature optimum, Topt. Traditionally enzyme temperature dependence has been modelled by the Arrhenius and Eyring-Polanyi equations. Deviations ... -
Characterisation of the FitAB Toxin-Antitoxin System in Neisseria gonorrhoeae
(The University of Waikato, 2019)Neisseria gonorrhoeae is the causative agent of the sexually transmitted infection, gonorrhoea. In a standard infection, N. gonorrhoeae invade epithelial cells lining the urogenital tract and exit into the sub-epithelial ... -
The structure and function of a bovine salivary protein - BSP30b
(The University of Waikato, 2019)The Bovine Salivary Protein 30b (BSP30b) is a member of the palate, lung, and nasal epithelium clone (PLUNC) family which includes human bactericidal/permeability-increasing protein (BPI) and lipopolysaccaride binding ... -
Mycobacterium tuberculosis strains in New Zealand: phylogeny and structural biology
(The University of Waikato, 2019)Mycobacterium tuberculosis is an obligate human pathogen and is the primary causative agent of tuberculosis. New Zealand has a relatively low incidence of tuberculosis disease, however, Māori (the indigenous people of New ... -
Do PhoH2 proteins regulate SigF in mycobacteria?
(The University of Waikato, 2018)There is little known about the role of the PhoH2 proteins in biology. PhoH2 consists of two domains, an N-terminal PIN domain with RNase activity and a C-terminal PhoH domain shown to have ATP induced RNA helicase activity. ... -
Evolution of Bacillus subtilis: A Novel Phenotype and its Challenges
(The University of Waikato, 2018)The enzyme LeuB from the last common ancestor of the firmicutes has been statistically recreated using several different methods. This project deals with two versions, Rec/LG and Rec/EX. To better understand these resurrected ... -
Engineering and characterisation of anti-progesterone OBodies
(The University of Waikato, 2018)Molecular interactions are fundamental to communication between different parts of the cell or of an organism. These interactions can be weak and transient or strong and semi-permanent. In the case of the adaptive immune ... -
Investigating Macromolecular Rate Theory
(The University of Waikato, 2017)Curvature with temperature is a defining characteristic of enzyme catalysed rates. Historically, curvature has been described by Arrhenius behaviour up to a temperature optimum (Tₒₚₜ), while decreases in rates above Tₒₚₜ ... -
Caldalkalibacillus thermarum PhoH2: Solving the solubility puzzle
(University of Waikato, 2017)Previous attempts to determine the structure of the protein PhoH2 from Mycobacterium tuberculosis, Mycobacterium smegmatis and Thermobispora bispora have been unsuccessful producing diffraction data to low resolution. The ... -
Testing Macromolecular Rate Theory
(University of Waikato, 2017)Enzymatic rate increase with respect to temperature has widely been described by transition-state theory. The experimentally observed rate decline above an optimum temperature (Tₒₚₜ) for enzymes has previously been attributed ... -
Regulation of Metabolism in Mycobacteria and Cyanobacteria
(University of Waikato, 2015)Bacteria encounter changes in their environment and must adapt to these changes in order to survive. Their ability to adapt is determined by their capacity to efficiently regulate their cellular processes. The mechanisms ... -
The evolution of AroA and MurA enzymes from Bacillus
(University of Waikato, 2016)MurA and AroA are important antibacterial targets due to their essentiality in microorganisms and the absence of their respective pathways within mammals. Although much research has focussed on these enzymes, little is ... -
Time to Diagnosis and Persistence: The Two Major Determinants of Effective Tuberculosis Control
(University of Waikato, 2015)The greatest challenge confronting effective tuberculosis (TB) eradication is the time to diagnosis, and duration of treatment of chronically infected individuals which represent a pool of infection. In an attempt to help ... -
Identifying the signalling pathway of a novel Myostatin Splice Variant (MSV)
(University of Waikato, 2014)Myostatin (Mstn), a member of the transforming growth factor-β super family, is a potent negative regulator of skeletal muscle mass. Studies delineating the function of Mstn have identified multiple signal transduction ... -
Characterisation of Enzyme Evolution through Ancestral Enzyme Reconstruction
(University of Waikato, 2013)Through ancestral sequence reconstruction (ASR) techniques, ancient enzymes can be recreated and biochemically tested, giving insight into the enzymes’ evolutionary history. A previous study by Hobbs et al. (2012) has ... -
Characterisation of VapBC Toxin-Antitoxins from Mycobacterium tuberculosis
(University of Waikato, 2013)Toxin-antitoxin (TA) systems were identified more than 20 years ago on the mini F plasmid of Escherichia coli as plasmid stability elements; components responsible for purging bacterial cells that lack the plasmid from the ...
