Browsing by Author "Arcus, Vickery L."

• Change in heat capacity for enzyme catalysis determines temperature dependence of enzyme catalyzed ratesnzyme Catalyzed Rates 

  Hobbs, Joanne K.; Jiao, Wanting; Easter, Ashley Davys; Parker, Emily J.; Schipper, Louis A.; Arcus, Vickery L. (American Chemical Society, 2013)

  The increase in enzymatic rates with temperature up to an optimum temperature (Topt) is widely attributed to classical Arrhenius behavior, with the decrease in enzymatic rates above Topt ascribed to protein denaturation ...

• Complete genome sequence of a New Zealand Mycobacterium tuberculosis strain responsible for ongoing transmission over the past 30 years. 

  Mulholland, Claire V.; Gimenez, Gregory; Williamson, Adele; Steele, Mackenzie; Thorpe, Duncan; Karalus, Noel; Cursons, Raymond T.; Playle, Veronica M.; Roberts, Sally A.; Cook, Gregory M.; Arcus, Vickery L.; Aung, Htin Lin (2022)

  We report here the complete genome sequence of Mycobacterium tuberculosis strain Colonial S-type 1 (CS1), which has been responsible for ongoing outbreaks of tuberculosis in New Zealand over the past 30 years. CS1 appears ...

  

• Crystal structure of an inferred ancestral bacterial pyruvate decarboxylase 

  Buddrus, Lisa; Andrews, Emma Sophie Vout; Leak, David J.; Danson, Michael J.; Arcus, Vickery L.; Crennell, Susan J. (International Union of Crystallography, 2018)

  Pyruvate decarboxylase (PDC; EC 4.1.1.1) is a key enzyme in homofermentative metabolism where ethanol is the major product. PDCs are thiamine pyrophos­phate- and Mg2+ ion-dependent enzymes that catalyse the non-oxidative ...

  

• Crystal structure of pyruvate decarboxylase from Zymobacter palmae 

  Buddrus, Lisa; Andrews, Emma Sophie Vout; Leak, David J.; Danson, Michael J.; Arcus, Vickery L.; Crennell, Susan J. (International Union of Crystallography, 2016)

  Pyruvate decarboxylase (PDC; EC 4.1.1.1) is a thiamine pyrophosphate- and Mg²⁺ ion-dependent enzyme that catalyses the non-oxidative decarboxylation of pyruvate to acetaldehyde and carbon dioxide. It is rare in bacteria, ...

  

• Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry 

  McKenzie, Joanna Leigh; Duyvestyn, Johanna Maria; Smith, Tony C.; Bendak, Katerina; Mackay, Joel P.; Cursons, Raymond T.; Cook, Gregory M.; Arcus, Vickery L. (RNA Society, 2012)

  The VapBC toxin-antitoxin (TA) family is the largest of nine identified TA families. The toxin, VapC, is a metal-dependent ribonuclease that is inhibited by its cognate antitoxin, VapB. Although the VapBCs are the largest ...

• Understanding enzymatic mechanism and allostery using macromolecular rate theory 

  Grout, Emily Katherine (The University of Waikato, 2022)

  Intricate systems of regulation at many levels act to control enzyme rates to tune metabolism across biosynthetic pathways. Allostery is a ubiquitous regulatory mechanism for feedback and regulation of enzymes in biosynthetic ...

  

• Verifying clustered mutation regions inside the newly-completed genome of a clinically-significant New Zealand Mycobacterium tuberculosis strain 

  Steele, Mackenzie (The University of Waikato, 2021)

  In 2019, active infections of Mycobacterium tuberculosis (Mtb) affected 10 million people globally, with 1.4 million deaths, and an additional 1.7-1.9 billion people infected without symptomatic disease (latent Mtb). While ...

  

• Measuring the temperature response of soil respiration from two distinct carbon pools in soil. 

  Robinson, Jasmine May (The University of Waikato, 2020)

  As soils contain a large store of terrestrial carbon, understanding the dynamics and stability of this important carbon reserve and how it may change with regards to future changes in temperature is of global interest. It ...

  

• Finding the good vibes of life, man: Identifying catalytic vibrations in enzyme catalysis 

  Schipper, Daniel-Albert (The University of Waikato, 2020)

  Enzyme catalysis is one of the most vital components of life. As such the elucidation of the exact mechanics of this kind of catalysis is important. The aim of this work is to look into enzyme catalysis though the lens of ...

  

• Understanding allosteric enzyme regulation using macromolecular rate theory 

  Hamill, Carlin James (The University of Waikato, 2020)

  Enzyme catalysed reaction rates produce a curved temperature dependence with a temperature optimum, Topt. Traditionally enzyme temperature dependence has been modelled by the Arrhenius and Eyring-Polanyi equations. Deviations ...

  

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