Browsing by Author "Danson, Michael J."

Now showing items 1-5 of 17

  • Assaying activity and assessing thermostability of hyperthermophilic enzymes

    Daniel, Roy M.; Danson, Michael J. (Elsevier, 2001)
    There is now a wide variety of intra- and extra-cellular enzymes available from organisms growing above 75°C, and having sufficient stability to allow assay well above this temperature. For some of these enzymes, to assay ...
  • Citrate synthases from the Archaea: Development of a bio-specific, affinity chromatography purification procedure

    James, Keith D.; Russell, Rupert J.M.; Parker, Lynne; Daniel, Roy M.; Hough, David W.; Danson, Michael J. (Wiley, 1994)
    Citrate synthases from both thermophilic and halophilic Archaea have been purified to homogeneity using affinity chromatography on Matrex Gel Red A and elution with a combination of substrate (oxaloacetate) and product ...
  • Crystal structure of an inferred ancestral bacterial pyruvate decarboxylase

    Buddrus, Lisa; Andrews, Emma Sophie Vout; Leak, David J.; Danson, Michael J.; Arcus, Vickery L.; Crennell, Susan J. (International Union of Crystallography, 2018)
    Pyruvate decarboxylase (PDC; EC 4.1.1.1) is a key enzyme in homofermentative metabolism where ethanol is the major product. PDCs are thiamine pyrophos­phate- and Mg2+ ion-dependent enzymes that catalyse the non-oxidative ...
  • Crystal structure of pyruvate decarboxylase from Zymobacter palmae

    Buddrus, Lisa; Andrews, Emma Sophie Vout; Leak, David J.; Danson, Michael J.; Arcus, Vickery L.; Crennell, Susan J. (International Union of Crystallography, 2016)
    Pyruvate decarboxylase (PDC; EC 4.1.1.1) is a thiamine pyrophosphate- and Mg²⁺ ion-dependent enzyme that catalyses the non-oxidative decarboxylation of pyruvate to acetaldehyde and carbon dioxide. It is rare in bacteria, ...
  • The dependence of enzyme activity on temperature: determination and validation of parameters

    Peterson, Michelle E.; Daniel, Roy M.; Danson, Michael J.; Eisenthal, Robert (Portland Press (Online), 2007)
    Traditionally, the dependence of enzyme activity on temperature has been described by a model consisting of two processes: the catalytic reaction defined by DGDaggercat, and irreversible inactivation defined by DGDaggerinact. ...