Browsing by Author "Monk, Colin R."
Now showing items 1-5 of 9
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Clostridium fervidus sp. nov., a New Chemoorganotrophic Acetogenic Thermophile
(Society for General Microbiology, 1987)Clostridium fervidus sp. nov. was isolated from a hot spring in New Zealand. The cells were strictly anaerobic, gram negative, sporeforming, and sluggishly motile rods (0.65 to 0.75 µ wide and 2 to 3 µm long). The spherical ... -
Determination of enzyme thermal parameters for rational enzyme engineering and environmental/ evolutionary studies
(Springer, 2013)Of the two independent processes by which enzymes lose activity with increasing temperature, irreversible thermal inactivation and rapid reversible equilibration with an inactive form, the latter is only describable by the ... -
Dynamics of immobilized and native Escherichia coli dihydrofolate reductase by quasielastic neutron scattering
(Biophysical Society, 2006-10)The internal dynamics of native and immobilized Escherichia coli dihydrofolate reductase (DHFR) have been examined using incoherent quasielastic neutron scattering. These results reveal no difference between the high ... -
The equilibrium model for the effect of temperature on enzymes: Insights and implications
(Teknoscienze SRL, 2008)A new, experimentally-validated “Equilibrium Model” describes the effect of temperature on enzymes, and provides a new mechanism for the reversible loss of enzyme activity with temperature. It incorporates two new, fundamental ... -
Esterase catalysis of substrate vapour: enzyme activity occurs at very low hydration
(Elsevier, 2004-10)It has been generally accepted that enzyme activity requires a minimal hydration of about 0.2 g H2O g⁻¹ protein. This fits well with evidence that hydration above this level is associated with the onset of intramolecular ...
