Browsing by Author "Petach, Helen H."
Now showing items 1-5 of 5
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Arginase from kiwifruit: properties and seasonal variation
(S I R publishing, 1997)The in vitro activity of arginase (EC 3.5.3.1) was investigated in youngest-mature leaves and roots (1-3 mm diameter) of kiwifruit vines (Actinidia deliciosa var. deliciosa) during an annual growth cycle, and enzyme from ... -
The effect of low temperatures on enzyme activity
(1995)The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain AN1 and beta-glucosidase from Caldocellum saccharolyticum expressed in Escherichia coli) has been exploited to allow ... -
A general method for the synthesis of peptidyl substrate for proteolytic enzymes
(Elsevier, 1995)The synthesis of an heterogeneous peptide substrate for the assay of proteases was carried out by cleaving a protein using a protease to generate small peptides which were then coupled to a chromophore, p-nitroaniline. The ... -
Properties and stabilization of an extracellular α-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN 1: enzyme activity at 130°C
(Elsevier, 1996)An extracellular α-glucosidase from the thermophilic archaebacterium Thermococcus strain AN1 was purified 875-fold in five steps (Hiload Q-Sepharose, phenyl Sepharose, HPHT-hydroxyapatite, gel filtration and Mono Q ... -
Review: The denaturation and degradation of stable enzymes at high temperatures
(1996)Now that enzymes are available that are stable above 100 °C it is possible to investigate conformational stability at this temperature, and also the effect of high-temperature degradative reactions in functioning enzymes ...
