Browsing by Author "Peterson, Michelle E."

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  • Cryosolvents useful for protein and enzyme studies below −100°C

    Reat, Valerie; Finney, John L.; Steer, Andrew; Roberts, Mark A.; Smith, Jeremy C.; Dunn, Rachel V.; Peterson, Michelle E.; Daniel, Roy M. (Elsevier, 2000)
    For the study of protein structure, dynamics, and function, at very low temperatures it is desirable to use cryosolvents that resist phase separation and crystallisation. We have examined these properties in a variety of ...
  • The dependence of enzyme activity on temperature: determination and validation of parameters

    Peterson, Michelle E.; Daniel, Roy M.; Danson, Michael J.; Eisenthal, Robert (Portland Press (Online), 2007)
    Traditionally, the dependence of enzyme activity on temperature has been described by a model consisting of two processes: the catalytic reaction defined by DGDaggercat, and irreversible inactivation defined by DGDaggerinact. ...
  • Eurythermalism and the temperature dependence of enzyme activity

    Lee, Charles Kai-Wu; Daniel, Roy M.; Shepherd, Charis; Saul, David; Cary, S. Craig; Danson, Michael J.; Eisenthal, Robert; Peterson, Michelle E. (Federation of American Societies for Experimental Biology, 2007)
    The "Equilibrium Model" has provided new tools for describing and investigating enzyme thermal adaptation. It has been shown that the effect of temperature on enzyme activity is not only governed by ΔG‡cat and ΔG‡inact but ...
  • Evidence for a third thermal parameter of enzymes

    Peterson, Michelle E. (The University of Waikato, 2005)
    This thesis describes tests of a new hypothesis describing the effect of temperature on enzyme activity. Traditionally, the dependence of enzyme activity on temperature has been described by a model (the 'Classical Model') ...
  • Matlab application for fitting progress curves to the Equilibrium Model

    Peterson, Michelle E.; McDowall, James; Goodhue, Nigel David; Bryan, Karin R.; Hailstone, Daniel; Monk, Colin R. (The University of Waikato, 2010)
    The general procedures for carrying out the necessary rate determinations required for accurate determination of the Equilibrium Model parameters, and fitting this data to the mathematical model to generate the parameters, ...