Now showing items 1-5 of 6

  • Cryosolvents useful for protein and enzyme studies below −100°C

    Reat, Valerie; Finney, John L.; Steer, Andrew; Roberts, Mark A.; Smith, Jeremy C.; Dunn, Rachel V.; Peterson, Michelle E.; Daniel, Roy M. (Elsevier, 2000)
    For the study of protein structure, dynamics, and function, at very low temperatures it is desirable to use cryosolvents that resist phase separation and crystallisation. We have examined these properties in a variety of ...
  • Enzyme activity and dynamics: xylanase activity in the absence of fast anharmonic dynamics

    Dunn, Rachel V.; Reat, Valerie; Finney, John L.; Ferrand, Michel; Smith, Jeremy C.; Daniel, Roy M. (2000)
    The activity and dynamics of a simple, single subunit enzyme, the xylanase from Thermotoga maritima strain Fj SS3B.1 have been measured under similar conditions, from -70 to +10 °C. The internal motions of the enzyme, as ...
  • Enzyme Dynamics and Activity: Time-Scale Dependence of Dynamical Transitions in Glutamate Dehydrogenase Solution

    Daniel, Roy M.; Finney, John L.; Reat, Valerie; Dunn, Rachel V.; Smith, Jeremy C. (1999-10)
    We have examined the temperature dependence of motions in a cryosolution of the enzyme glutamate dehydrogenase (GDH) and compared these with activity. Dynamic neutron scattering was performed with two instruments of different ...
  • Nanosecond protein dynamics: First detection of a neutron incoherent spin−echo signal

    Bellissent-Funel, Marie-Claire; Daniel, Roy M.; Durand, Dominique; Ferrand, Michel; Finney, John L.; Pouget, Stephanie; Reat, Valerie; Smith, Jeremy C. (American Chemical Society, 1998)
    Motions in proteins occur over a wide range of time scales, from femtoseconds to seconds or longer.¹⁻³ Therefore, their characterization requires the application of a correspondingly wide range of experimental probes. Among ...
  • Solvent dependence of dynamic transitions in protein solutions

    Reat, Valerie; Dunn, Rachel V.; Ferrand, Michel; Finney, John L.; Daniel, Roy M.; Smith, Jeremy C. (2000)
    A transition as a function of increasing temperature from harmonic to anharmonic dynamics has been observed in globular proteins by using spectroscopic, scattering, and computer simulation techniques. We present here results ...