Browsing by Author "Smith, Jeremy C."

Now showing items 1-5 of 17

  • Cryosolvents useful for protein and enzyme studies below −100°C

    Reat, Valerie; Finney, John L.; Steer, Andrew; Roberts, Mark A.; Smith, Jeremy C.; Dunn, Rachel V.; Peterson, Michelle E.; Daniel, Roy M. (Elsevier, 2000)
    For the study of protein structure, dynamics, and function, at very low temperatures it is desirable to use cryosolvents that resist phase separation and crystallisation. We have examined these properties in a variety of ...
  • Direct determination of vibrational density of states change on ligand binding to a protein

    Balog, Erika; Becker, Torsten; Oettl, Martin; Lechner, Ruep; Daniel, Roy M.; Finney, John L.; Smith, Jeremy C. (American Physical Society, 2004)
    The change in the vibrational density of states of a protein (dihydrofolate reductase) on binding a ligand (methotrexate) is determined using inelastic neutron scattering. The vibrations of the complex soften significantly ...
  • The dynamic transition in proteins may have a simple explanation

    Daniel, Roy M.; Finney, John L.; Smith, Jeremy C. (Royal Society of Chemistry, 2003)
    The transition that has been observed in the dynamics of hydrated proteins at low temperatures (180–230 K) is normally interpreted as a change from vibrational, harmonic motion at low temperatures to anharmonic motions as ...
  • Dynamics of immobilized and native Escherichia coli dihydrofolate reductase by quasielastic neutron scattering

    Tehei, Moeava; Smith, Jeremy C.; Monk, Colin R.; Ollivier, Jacques; Oettl, Martin; Kurkal, Vandana; Finney, John L.; Daniel, Roy M. (Biophysical Society, 2006-10)
    The internal dynamics of native and immobilized Escherichia coli dihydrofolate reductase (DHFR) have been examined using incoherent quasielastic neutron scattering. These results reveal no difference between the high ...
  • Enzyme activity and dynamics: xylanase activity in the absence of fast anharmonic dynamics

    Dunn, Rachel V.; Reat, Valerie; Finney, John L.; Ferrand, Michel; Smith, Jeremy C.; Daniel, Roy M. (2000)
    The activity and dynamics of a simple, single subunit enzyme, the xylanase from Thermotoga maritima strain Fj SS3B.1 have been measured under similar conditions, from -70 to +10 °C. The internal motions of the enzyme, as ...