Browsing by Supervisor "Oback, Bjorn"
Now showing items 1-8 of 8
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Deriving bovine embryonic stem-like cells in defined conditions
(University of Waikato, 2011)The first embryonic stem ES cell line was isolated from mouse in 1981 (Evans and Kaufman 1981; Martin1981). However, ESCs are only available in rodent species. Although research has been carried out on bovine embryos for ... -
Doxycycline-inducible overexpression of NANOG in bovine fibroblasts and nuclear transfer embryos
(University of Waikato, 2015)Naïve pluripotent embryonic stem cells (ES cells) have the ability to give rise to all cell types including functional gametes. These cells have been well established in mice and rats. The potential for biomedical and ... -
Improving bovine donor cell culture conditions for embryo-derived cell transfer cloning
(The University of Waikato, 2021)Cloning by cell transfer offers an efficient method for increasing genetic gain in livestock populations. High-quality genes are routinely induced into embryos with artificial selection-based in vitro fertilisation, or ... -
Inhibition of ERK and GSK3 signalling pathways promotes development and expression of pluripotent markers in bovine blastocysts
(University of Waikato, 2012)Embryo-derived pluripotent stem cells (ePSCs) naturally derive from different founder tissues, namely the early and late epiblast, that vary in their cellular characteristics. Recent evidence in mice suggests these sources ... -
Optimized vitrification, cell cycle compatibility and volume of cytoplasts for bovine embryonic cloning
(The University of Waikato, 2022)Cell transfer cloning is a promising method for multiplying elite alleles in a population. Together with gene editing, it can increase the genetic gain of livestock and support the adaptation of cattle to climate change. ... -
Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
(University of Waikato, 2013)Cloning by somatic cell nuclear transfer (SCNT) forces cells to lose their lineage-specific epigenetic marks and become totipotent again. This reprogramming process often results in epigenetic and transcriptional aberrations ... -
The Role of Amino Acids and the Threonine-SAM Pathway in the Development of Bovine Inner Cell Mass and Pluripotency
(The University of Waikato, 2018)Pluripotent stem cells (PSCs) are the foundation of all cell types in the body including functional gametes. They are derived either from the inner cell mass (ICM) of preimplantation mouse and rat embryos, known as embryonic ... -
Using putative bovine embryonic stem cell-like cells for nuclear transfer cloning to determine their functional potential
(University of Waikato, 2015)The potential for cloning to improve dissemination of elite genetics has been a goal of livestock scientists for many years. However, efficiency of this technique has remained low compared to other in vitro embryo production ...
