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dc.contributor.authorBanks, Jonathan C.en_NZ
dc.contributor.authorHogg, Ian D.en_NZ
dc.date.accessioned2016-05-30T03:13:43Z
dc.date.available2014en_NZ
dc.date.available2016-05-30T03:13:43Z
dc.date.issued2014en_NZ
dc.identifier.citationBanks, J. C., & Hogg, I. D. (2014). Development and validation of hydrolysis assays for seven species of exotic fish (ERI report No. 44) (pp. 1–17). Client report prepared for Lake Ecosystem Restoration New Zealand. Hamilton, New Zealand: Environmental Research Institute, The University of Waikato.en
dc.identifier.urihttps://hdl.handle.net/10289/10282
dc.description.abstractDetermining the distribution and presence of exotic taxa are significant challenges for eradication efforts and for restoration ecology. One method is detect the presence of introduced species that has shown promise is the use of environmental DNA-DNA obtained from an environmental sample such as soil or sediment rather than directly from a tissue sample. As part of Intermediate Output 2 (IO2) of the Lake Ecosystem Restoration New Zealand project, we were asked to develop hydrolysis assays (Commonly called Taqman assays) to detect the presence of seven species of introduced fish. Here we report the sequences of the primers and the primers and the hydrolysis probe for assays for catfish, Ameiurus nebulosus; mosquito fish, Gambusia affinis; goldfish, Carassius auratus; Koi carp, Cyprinus carpio; perch Perca fluviatilis; rudd, Scardinius erythrophthalmus and tench, Tinca tinca. We identified assay conditions that resulted in true-negative results by testing for cross reactivity against the six species of exotic fish that were not the target species. Additionally, we compared the effect of adding different amounts of DNA to the found that adding more DNA template can result in false-negative results (DNA present but not detected) possibly due to the concentration of inhibitory substances reaching concentrations high enough to inhibit the assays.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherEnvironmental Research Institute, The University of Waikato.en_NZ
dc.relation.ispartofseriesERI report
dc.rights© 2014 the authors.
dc.titleDevelopment and validation of hydrolysis assays for seven species of exotic fishen_NZ
dc.typeTechnical Report
uow.relation.series44
pubs.begin-page1
pubs.commissioning-bodyLake Ecosystem Restoration New Zealanden_NZ
pubs.confidentialfalseen_NZ
pubs.elements-id128157
pubs.end-page17


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