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dc.contributor.authorSummers, Emma Louiseen_NZ
dc.contributor.authorMeindl, Kathrinen_NZ
dc.contributor.authorUsón, Isabelen_NZ
dc.contributor.authorMitra, Alok K.en_NZ
dc.contributor.authorRadjainia, Mazdaken_NZ
dc.contributor.authorColangeli, Robertoen_NZ
dc.contributor.authorAlland, Daviden_NZ
dc.contributor.authorArcus, Vickery L.en_NZ
dc.date.accessioned2016-11-04T01:20:01Z
dc.date.available2012-06-13en_NZ
dc.date.available2016-11-04T01:20:01Z
dc.date.issued2012-06-13en_NZ
dc.identifier.citationSummers, E. L., Meindl, K., Usón, I., Mitra, A. K., Radjainia, M., Colangeli, R., … Arcus, V. L. (2012). The Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protection. PLoS ONE, 7(6): e38542. http://doi.org/10.1371/journal.pone.0038542en
dc.identifier.issn1932-6203en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/10707
dc.description.abstractLsr2 is a small DNA-binding protein present in mycobacteria and related actinobacteria that regulates gene expression and influences the organization of bacterial chromatin. Lsr2 is a dimer that binds to AT-rich regions of chromosomal DNA and physically protects DNA from damage by reactive oxygen intermediates (ROI). A recent structure of the C-terminal DNAbinding domain of Lsr2 provides a rationale for its interaction with the minor groove of DNA, its preference for AT-rich tracts, and its similarity to other bacterial nucleoid-associated DNA-binding domains. In contrast, the details of Lsr2 dimerization (and oligomerization) via its N-terminal domain, and the mechanism of Lsr2-mediated chromosomal crosslinking and protection is unknown. We have solved the structure of the N-terminal domain of Lsr2 (N-Lsr2) at 1.73 A˚ resolution using crystallographic ab initio approaches. The structure shows an intimate dimer of two ß–ß–a motifs with no close homologues in the structural databases. The organization of individual N-Lsr2 dimers in the crystal also reveals a mechanism for oligomerization. Proteolytic removal of three N-terminal residues from Lsr2 results in the formation of an anti-parallel b-sheet between neighboring molecules and the formation of linear chains of N-Lsr2. Oligomerization can be artificially induced using low concentrations of trypsin and the arrangement of N-Lsr2 into long chains is observed in both monoclinic and hexagonal crystallographic space groups. In solution, oligomerization of N-Lsr2 is also observed following treatment with trypsin. A change in chromosomal topology after the addition of trypsin to full-length Lsr2-DNA complexes and protection of DNA towards DNAse digestion can be observed using electron microscopy and electrophoresis. These results suggest a mechanism for oligomerization of Lsr2 via protease-activation leading to chromosome compaction and protection, and concomitant down-regulation of large numbers of genes. This mechanism is likely to be relevant under conditions of stress where cellular proteases are known to be upregulated.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherPLOSen_NZ
dc.rights© 2012 Summers et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectScience & Technologyen_NZ
dc.subjectMultidisciplinary Sciencesen_NZ
dc.subjectScience & Technology - Other Topicsen_NZ
dc.subjectMULTIDISCIPLINARY SCIENCESen_NZ
dc.subjectHISTONE-LIKE PROTEINen_NZ
dc.subjectNUCLEOID-ASSOCIATED PROTEINSen_NZ
dc.subjectDNAen_NZ
dc.subjectSMEGMATISen_NZ
dc.subjectLEPRAEen_NZ
dc.subjectRESPONSESen_NZ
dc.subjectSOFTWAREen_NZ
dc.subjectGENOMESen_NZ
dc.subjectGENEen_NZ
dc.titleThe Structure of the Oligomerization Domain of Lsr2 from Mycobacterium tuberculosis Reveals a Mechanism for Chromosome Organization and Protectionen_NZ
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0038542en_NZ
dc.relation.isPartOfPLoS ONEen_NZ
pubs.declined2016-11-04T08:59:09.799+1300
pubs.deleted2016-11-04T08:59:09.799+1300
pubs.elements-id22409
pubs.issue6en_NZ
pubs.publication-statusPublisheden_NZ
pubs.volume7en_NZ
uow.identifier.article-noe38542


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