Research Commons
      • Browse 
        • Communities & Collections
        • Titles
        • Authors
        • By Issue Date
        • Subjects
        • Types
        • Series
      • Help 
        • About
        • Collection Policy
        • OA Mandate Guidelines
        • Guidelines FAQ
        • Contact Us
      • My Account 
        • Sign In
        • Register
      View Item 
      •   Research Commons
      • University of Waikato Research
      • Science and Engineering
      • Science and Engineering Papers
      • View Item
      •   Research Commons
      • University of Waikato Research
      • Science and Engineering
      • Science and Engineering Papers
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark (Scyliorhinus canicula)

      Li, Ronggai; Redmond, Anthony K.; Wang, Tiehui; Bird, Steve; Dooley, Helen; Secombes, Chris J.
      Thumbnail
      Files
      Characterisation of the TNF superfamily accepted manuscript.pdf
      Accepted version, 2.920Mb
      DOI
       10.1016/j.fsi.2015.09.033
      Find in your library  
      Citation
      Export citation
      Li, R., Redmond, A. K., Wang, T., Bird, S., Dooley, H., & Secombes, C. J. (2015). Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark (Scyliorhinus canicula). Fish & Shellfish Immunology, 47(1), 381–389. https://doi.org/10.1016/j.fsi.2015.09.033
      Permanent Research Commons link: https://hdl.handle.net/10289/10966
      Abstract
      The tumour necrosis factor superfamily (TNFSF) members CD40L and BAFF play critical roles in mammalian B cell survival, proliferation and maturation, however little is known about these key cytokines in the oldest jawed vertebrates, the cartilaginous fishes. Here we report the cloning of CD40L and BAFF orthologues (designated ScCD40L and ScBAFF) in the small-spotted catshark (Scyliorhinus canicula). As predicted both proteins are type II membrane-bound proteins with a TNF homology domain in their extracellular region and both are highly expressed in shark immune tissues. ScCD40L transcript levels correlate with those of TCRα and transcription of both genes is modulated in peripheral blood leukocytes following in vitro stimulation. Although a putative CD40L orthologue was identified in the elephant shark genome the work herein is the first molecular characterisation and transcriptional analysis of CD40L in a cartilaginous fish. ScBAFF was also cloned and its transcription characterised in an attempt to resolve the discrepancies observed between spiny dogfish BAFF and bamboo shark BAFF in previously published studies.
      Date
      2015-11
      Type
      Journal Article
      Rights
      This is an author’s accepted version of an article published in the journal: Fish & Shellfish Immunology. Copyright © 2015 Elsevier Ltd.
      Collections
      • Science and Engineering Papers [3124]
      Show full item record  

      Usage

      Downloads, last 12 months
      92
       
       
       

      Usage Statistics

      For this itemFor all of Research Commons

      The University of Waikato - Te Whare Wānanga o WaikatoFeedback and RequestsCopyright and Legal Statement