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dc.contributor.authorHoughton, Karen M.en_NZ
dc.contributor.authorMorgan, Xochitl C.en_NZ
dc.contributor.authorLagutin, Kirillen_NZ
dc.contributor.authorMacKenzie, Andrew D.en_NZ
dc.contributor.authorVyssotskii, Mikhailen_NZ
dc.contributor.authorMitchell, Kevin A.en_NZ
dc.contributor.authorMcDonald, Ian R.en_NZ
dc.contributor.authorMorgan, Hugh W.en_NZ
dc.contributor.authorPower, Jean F.en_NZ
dc.contributor.authorMoreau, John W.en_NZ
dc.contributor.authorHanssen, Ericen_NZ
dc.contributor.authorStott, Matthew B.en_NZ
dc.date.accessioned2018-05-02T04:40:45Z
dc.date.available2015-12-01en_NZ
dc.date.available2018-05-02T04:40:45Z
dc.date.issued2015en_NZ
dc.identifier.citationHoughton, K. M., Morgan, X. C., Lagutin, K., MacKenzie, A. D., Vyssotskii, M., Mitchell, K. A., … Stott, M. B. (2015). Thermorudis pharmacophila sp nov., a novel member of the class Thermomicrobia isolated from geothermal soil, and emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus. International Journal of Systematic and Evolutionary Microbiology, 65, 4479–4487. https://doi.org/10.1099/ijsem.0.000598en
dc.identifier.issn1466-5026en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/11806
dc.description.abstractAn aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2T grew at 53–76 °C and at pH 5.9–8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C18 : 0 and C18 : 0. Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1-O-phosphoinositol (diolPI), 2-acylalkyldiol-1-O-phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1-O-phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1-O-phosphoinositol mannoside (diolPI-Man). Strain WKT50.2T utilized a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilize monosaccharides. Robust growth of WKT50.2T was observed on protein derivatives. WKT50.2T was sensitive to ampicillin, chloramphenicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analysis of 16S rRNA gene sequences showed that WKT50.2T belonged to the class Thermomicrobia within the phylum Chloroflexi, and was most closely related to Thermorudis peleae KI4T (99.6% similarity). DNA–DNA hybridization between WKT50.2T and Thermorudis peleae DSM 27169T was 18.0%. Physiological and biochemical tests confirmed the phenotypic and genotypic differentiation of strain WKT50.2T from Thermorudis peleae KI4T and other members of the Thermomicrobia. On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain WKT50.2T represents a novel species, for which the name Thermorudis pharmacophila sp. nov. is proposed, with the type strain WKT50.2T ( = DSM 26011T = ICMP 20042T). Emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilus are also proposed, and include the description of a novel respiratory quinone, MK-8 2,3-epoxide (23%), in Thermomicrobium roseum.
dc.format.mimetypeapplication/pdf
dc.language.isoenen_NZ
dc.publisherMicrobiology Societyen_NZ
dc.rightsThis is an author’s accepted version of an article published in the journal: International Journal of Systematic and Evolutionary Microbiology. © 2015 IUMS Institute of Geological and Nuclear Sciences (GND), New Zealand.
dc.subjectScience & Technologyen_NZ
dc.subjectLife Sciences & Biomedicineen_NZ
dc.subjectMicrobiologyen_NZ
dc.subjectNITRITE-OXIDIZING BACTERIUMen_NZ
dc.subjectCOMPLETE GENOME SEQUENCEen_NZ
dc.subjectPHYLUM CHLOROFLEXIen_NZ
dc.subjectGEN. NOV.en_NZ
dc.subjectTHERMOBACULUM-TERRENUMen_NZ
dc.subjectMICROBIAL DIVERSITYen_NZ
dc.subjectRAPID METHODen_NZ
dc.subjectHOT-SPRINGSen_NZ
dc.subjectNEW-ZEALANDen_NZ
dc.subjectPEPTIDOGLYCANen_NZ
dc.titleThermorudis pharmacophila sp nov., a novel member of the class Thermomicrobia isolated from geothermal soil, and emended descriptions of Thermomicrobium roseum, Thermomicrobium carboxidum, Thermorudis peleae and Sphaerobacter thermophilusen_NZ
dc.typeJournal Article
dc.identifier.doi10.1099/ijsem.0.000598en_NZ
dc.relation.isPartOfInternational Journal of Systematic and Evolutionary Microbiologyen_NZ
pubs.begin-page4479
pubs.elements-id130168
pubs.end-page4487
pubs.publication-statusPublisheden_NZ
pubs.volume65en_NZ
dc.identifier.eissn1466-5034en_NZ


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