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dc.contributor.authorJacobsen, Carsten Suhren_NZ
dc.contributor.authorNielsen, Tue Kjærgaarden_NZ
dc.contributor.authorVester, Jan Kjølhedeen_NZ
dc.contributor.authorStougaard, Peteren_NZ
dc.contributor.authorNielsen, Jeppe Lunden_NZ
dc.contributor.authorVoriskova, Janaen_NZ
dc.contributor.authorWinding, Anneen_NZ
dc.contributor.authorBaldrian, Petren_NZ
dc.contributor.authorLiu, Binbinen_NZ
dc.contributor.authorFrostegard, Åsaen_NZ
dc.contributor.authorPedersen, Dortheen_NZ
dc.contributor.authorTveit, Alexander Tøsdalen_NZ
dc.contributor.authorSvenning, Mette Marianneen_NZ
dc.contributor.authorTebbe, Christoph C.en_NZ
dc.contributor.authorOvreas, Lise Øvreåsen_NZ
dc.contributor.authorJakobsen, Pia Bachen_NZ
dc.contributor.authorBlazewicz, Steven J.en_NZ
dc.contributor.authorHubablek, Valerieen_NZ
dc.contributor.authorBertilsson, Stefanen_NZ
dc.contributor.authorHansen, Lars Hestbjergen_NZ
dc.contributor.authorCary, S. Craigen_NZ
dc.contributor.authorHolben, William E.en_NZ
dc.contributor.authorEkelund, Flemmingen_NZ
dc.contributor.authorBaelum, Jacoben_NZ
dc.date.accessioned2018-06-06T23:40:24Z
dc.date.available2018-04-09en_NZ
dc.date.available2018-06-06T23:40:24Z
dc.date.issued2018-04-09en_NZ
dc.identifier.citationJacobsen, C. S., Nielsen, T. K., Vester, J. K., Stougaard, P., Nielsen, J. L., Voriskova, J., … Baelum, J. (2018). Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples. Scientific Reports, 8. https://doi.org/10.1038/s41598-018-24082-yen
dc.identifier.issn2045-2322en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/11882
dc.description.abstractHere we show that a commercial blocking reagent (G2) based on modifed eukaryotic DNA signifcantly improved DNA extraction efciency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the number of PCR-amplifed16S rRNA genes recovered from biomass naturally present in the soils by one log unit. An extensive sequencing approach demonstrated that the blocking reagent was free of contaminating DNA, and may therefore also be used in metagenomics studies that require direct sequencing.
dc.format.mimetypeapplication/pdf
dc.language.isoenen_NZ
dc.publisherNature Publishing Groupen_NZ
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.subjectScience & Technologyen_NZ
dc.subjectMultidisciplinary Sciencesen_NZ
dc.subjectScience & Technology - Other Topicsen_NZ
dc.subjectSOIL BACTERIALen_NZ
dc.subjectRIBOSOMAL-RNAen_NZ
dc.subjectPLASMID DNAen_NZ
dc.subjectADSORPTIONen_NZ
dc.subjectCOMMUNITYen_NZ
dc.subjectCOEXTRACTIONen_NZ
dc.subjectGENEen_NZ
dc.titleInter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samplesen_NZ
dc.typeJournal Article
dc.identifier.doi10.1038/s41598-018-24082-yen_NZ
dc.relation.isPartOfScientific Reportsen_NZ
pubs.elements-id221759
pubs.organisational-group/Waikato
pubs.organisational-group/Waikato/2018 PBRF
pubs.organisational-group/Waikato/FSEN
pubs.organisational-group/Waikato/FSEN/2018 PBRF - FSEN
pubs.organisational-group/Waikato/FSEN/School of Science
pubs.organisational-group/Waikato/FSEN/School of Science/2018 PBRF - School of Science
pubs.publication-statusPublisheden_NZ
pubs.volume8en_NZ
uow.identifier.article-noARTN 5711


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