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dc.contributor.authorRego, Adrianaen_NZ
dc.contributor.authorRaio, Franciscoen_NZ
dc.contributor.authorMartins, Teresa P.en_NZ
dc.contributor.authorRibeiro, Hugoen_NZ
dc.contributor.authorSousa, António G. G.en_NZ
dc.contributor.authorSilva, Joanaen_NZ
dc.contributor.authorBaptista, Mafalda S.en_NZ
dc.contributor.authorLee, Charles Kai-Wuen_NZ
dc.contributor.authorCary, S. Craigen_NZ
dc.contributor.authorRamos, Vitoren_NZ
dc.contributor.authorCarvalho, Maria F.en_NZ
dc.contributor.authorLeão, Pedro N.en_NZ
dc.contributor.authorMagalhães, Catarinaen_NZ
dc.date.accessioned2019-06-04T00:38:37Z
dc.date.available2019en_NZ
dc.date.available2019-06-04T00:38:37Z
dc.date.issued2019en_NZ
dc.identifier.citationRego, A., Raio, F., Martins, T. P., Ribeiro, H., Sousa, A. G. G., Silva, J., … Magalhães, C. (2019). Actinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments using culture-dependent and independent methods. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2019.01018en
dc.identifier.issn1664-302Xen_NZ
dc.identifier.urihttps://hdl.handle.net/10289/12589
dc.description.abstractBacterial diversity from McMurdo Dry Valleys in Antarctica, the largest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains unachievable by the power of sequencing. In this study, we combine cultivation and HTS techniques to survey Actinobacteria and Cyanobacteria diversity along different soil and endolithic micro-environments of Victoria Valley in McMurdo Dry Valleys. Our results have demonstrated that the Dry Valleys Actinobacteria and Cyanobacteria distribution is driven by environmental forces, in particular the effect of water availability and endolithic environments clearly conditioned the distribution of those communities. Data derived from HTS show that the percentage of Cyanobacteria decreases from about 20% in the sample closest to the water source to neglectable values on the last three samples of the transect with less water availability. Inversely, Actinobacteria abundance increases from the wet to the driest samples. Over 30% of the total HTS data set was composed of Actinobacterial strains, mainly distributed by 5 families: Sporichthyaceae, Euzebyaceae, Patulibacteraceae, Nocardioidaceae and Rubrobacteraceae. However, the 11 actinobacterial strains isolated in this study, belonged to Micrococcaceae and Dermacoccaceae families that were underrepresented in the HTS data set. A total of 10 cyanobacterial strains from the order Synechococcales were also isolated, distributed by 4 different genera (Nodosilinea, Leptolyngbya, Pectolyngbya, Acaryochloris). In agreement with the cultivation results, Lepyolyngbya was identified as dominant genera in the HTS data set. Acaryochloris genus were found exclusively in the endolithic sample and represented 44% of the total 16S rDNA sequences, although despite our efforts we were not able to properly isolate any strain from Acaryochloris genus. The importance of combining cultivation and sequencing techniques is highlighted, as we have shown that culture-dependent methods employed in this study were able to retrieve Actinobacteria and Cyanobacteria taxa that were not detected in HTS data set, suggesting that the combination of both strategies can be usefull to recover both abundant and rare members of the communities.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherFrontiers Mediaen_NZ
dc.rightsCopyright © 2019 Rego, Raio, Martins, Ribeiro, Sousa, Séneca, Baptista, Lee, Cary, Ramos, Carvalho, Leão and Magalhães. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.subjectActinobacteriaen_NZ
dc.subjectMcMurdo Dry Valleysen_NZ
dc.subjectAntarctic soilen_NZ
dc.subjectBacteria diversityen_NZ
dc.subjectBacterial cultivabilityen_NZ
dc.subjectEndolitic microbiotaen_NZ
dc.subjectAntarctic microenvironmentsen_NZ
dc.subjectCyanobacteriaen_NZ
dc.titleActinobacteria and cyanobacteria diversity in terrestrial Antarctic microenvironments using culture-dependent and independent methodsen_NZ
dc.typeJournal Article
dc.identifier.doi10.3389/fmicb.2019.01018en_NZ
dc.relation.isPartOfFrontiers in Microbiologyen_NZ
pubs.elements-id237515
pubs.publication-statusAccepteden_NZ
pubs.publisher-urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01018/abstracten_NZ


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