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      Dynamics of immobilized and native Escherichia coli dihydrofolate reductase by quasielastic neutron scattering

      Tehei, Moeava; Smith, Jeremy C.; Monk, Colin R.; Ollivier, Jacques; Oettl, Martin; Kurkal, Vandana; Finney, John L.; Daniel, Roy M.
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      Biophy Immobilized.pdf
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      DOI
       10.1529/biophysj.105.062182
      Link
       www.biophysj.org
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      Tehei, M., Smith, J.C., Monk, C., Ollivier, J., Oettl, M., Kurkal, V., Finney, J. L. & Daniel, R.M. (2006). Dynamics of immobilized and native Escherichia coli dihydrofolate reductase by quasielastic neutron scattering. Biophysical Journal, 90, 1090- 1097.
      Permanent Research Commons link: https://hdl.handle.net/10289/1288
      Abstract
      The internal dynamics of native and immobilized Escherichia coli dihydrofolate reductase (DHFR) have been examined using incoherent quasielastic neutron scattering. These results reveal no difference between the high frequency vibration mean-square displacement of the native and the immobilized E. coli DHFR. However, length-scale-dependent, picosecond dynamical changes are found. On longer length scales, the dynamics are comparable for both DHFR samples. On shorter length scales, the dynamics is dominated by local jump motions over potential barriers. The residence time for the protons to stay in a potential well is = 7.95 ± 1.02 ps for the native DHFR and = 20.36 ± 1.80 ps for the immobilized DHFR. The average height of the potential barrier to the local motions is increased in the immobilized DHFR, and may increase the activation energy for the activity reaction, decreasing the rate as observed experimentally. These results suggest that the local motions on the picosecond timescale may act as a lubricant for those associated with DHFR activity occurring on a slower millisecond timescale. Experiments indicate a significantly slower catalytic reaction rate for the immobilized E. coli DHFR. However, the immobilization of the DHFR is on the exterior of the enzyme and essentially distal to the active site, thus this phenomenon has broad implications for the action of drugs distal to the active site.
      Date
      2006-10
      Type
      Journal Article
      Publisher
      Biophysical Society
      Rights
      This article has been published in the journal: Biophysical Journal. Copyright © 2006 by the Biophysical Society.
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