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dc.contributor.advisorPeters, Linda M.
dc.contributor.authorCecire, Anthony
dc.date.accessioned2021-01-13T19:50:07Z
dc.date.available2021-01-13T19:50:07Z
dc.date.issued2020
dc.identifier.citationCecire, A. (2020). Investigation of respiratory surfactant A protein in middle ear epithelium (Thesis, Master of Philosophy (MPhil)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/14055en
dc.identifier.urihttps://hdl.handle.net/10289/14055
dc.description.abstractIntroduction: The middle ear of mammals is an air-chamber required for sound transmission. It is lined by an epithelium which is variable but which resembles that of the lungs. Lung function depends upon surfactant which is a family of surfactant proteins which have an important role in breathing as well as infection control. Given that both the middle ear and the lungs can suffer collapse as well as infection, it is worth exploring whether surfactant protein is expressed throughout the middle ear in any manner analogous to that in the lung. Materials and methods: Our study has used simple histological stains to study cell and tissue morphology of the rodent middle ear. We used immunohistochemistry and protein electrophoresis to identify surfactant A protein as well as four cytokeratins expressed in lung cells. Results: We have demonstrated that surfactant protein A is indeed expressed in the middle ear and that there are cells which have a protein cytokeratin expression consistent with surfactant production and other cells whose cytokeratin expression reflects a likely role in gas exchange. Our research has also confirmed the presence of surfactant in the bone marrow spaces adjacent to the middle ear as well as other tissues reflecting its diverse roles. This also suggests a role in local and innate immunity. A novel finding was the presence of communications between the marrow spaces and the mouse middle ear cavity. Conclusion: These results suggest that there is a need for further work to assess the anatomical and topographical distribution of surfactant A protein throughout the variety of cell types within the middle ear. This will help to understand better the interaction of the various cell types in regard to the local and innate immunity of the middle ear as well as its gas exchange function.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherThe University of Waikato
dc.rightsAll items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectSurfactant A protein
dc.subjectMiddle ear
dc.titleInvestigation of respiratory surfactant A protein in middle ear epithelium
dc.typeThesis
thesis.degree.grantorThe University of Waikato
thesis.degree.levelDoctoral
thesis.degree.nameMaster of Philosophy (MPhil)
dc.date.updated2020-12-18T00:35:35Z
pubs.place-of-publicationHamilton, New Zealanden_NZ


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