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dc.contributor.authorSu, Chia-Hung
dc.contributor.authorFu, Chun-Chong
dc.contributor.authorChang, Yet-Chung
dc.contributor.authorNair, Giridhar R.
dc.contributor.authorYe, Jun-Liang
dc.contributor.authorChu, I-Ming
dc.contributor.authorWu, Wen-Teng
dc.date.accessioned2009-07-08T03:19:25Z
dc.date.available2009-07-08T03:19:25Z
dc.date.issued2008
dc.identifier.citationSu, C.-H., Fu, C.-C., Chang, Y.-C., Nair, G.R., Ye, J.-L., Chu, I.-M. & Wu, W.-T. (2007). Simultaneous estimation of chlorophyll a and lipid contents in microalgae by three-color analysis. Biotechnology and Bioengineering, 99(4), 1034-1039.en
dc.identifier.urihttps://hdl.handle.net/10289/2220
dc.description.abstractA method of rapid determination of chlorophyll a and lipid contents of microalgae based on colorimetric analysis of the digital images of the microalgae is proposed. The color variation of microalgae during cultivation is evaluated by the brightness of the three primary colors (red, green, and blue). The brightness values of the three primary colors are modeled as two linear correlation functions (RGB model) for microalgal chlorophyll a and lipid contents, respectively. The chlorophyll a and lipid contents predicted by the proposed model are compared with that determined by the standard methods. The good agreement of the model predictions with experimental results is demonstrated with a squared correlation coefficient (R2) of 0.99 for chlorophyll a and lipid. The reliability of the RGB model was verified in real cultivations of the microalgae in a photobioreactor. Growth dynamics, contents of chlorophyll a and lipid corresponded very well with previously reported studies.en
dc.language.isoen
dc.publisherWileyen_NZ
dc.subjectmicroalgaeen
dc.subjectimage analysisen
dc.subjectchlorophyllen
dc.subjectlipiden
dc.subjectRGB modelen
dc.titleSimultaneous estimation of chlorophyll a and lipid contents in microalgae by three-color analysisen
dc.typeJournal Articleen
dc.identifier.doi10.1002/bit.21623en
dc.relation.isPartOfBiotechnology and Bioengineeringen_NZ
pubs.begin-page1034en_NZ
pubs.elements-id34112
pubs.end-page1039en_NZ
pubs.issue4en_NZ
pubs.volume99en_NZ


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