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dc.contributor.authorWu, Kevin Su-Weien_NZ
dc.date.accessioned2007-02-23T23:23:38Z
dc.date.available2009-06-12T01:01:01Z
dc.date.issued2007en_NZ
dc.identifier.citationWu, K. S.-W. (2007). Preparation of Stable Gold Colloids for Sensitivity Enhancement of Progesterone Immunoassay using Surface Plasmon Resonance (Thesis, Master of Science (MSc)). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/2279en
dc.identifier.urihttps://hdl.handle.net/10289/2279
dc.description.abstractThe purpose of this study was to prepare concentrated and stable gold colloids for the enhancement of the signal response of the SPR technique for detecting small molecules such as progesterone. The gold colloids developed in this study were prepared by hydrazine hydrate, sodium borohydride, and tri-potassium citrate reduction routes. The study revealed that the sodium borohydride reduced gold colloids were extremely stable and it was able to be utilised in the progesterone immunoassay developed previously by Mitchell et al. The experiment was carried out on BIAcore 3000 using two different sensor surfaces (CM5 and SAM). The results showed that the enhancement species prepared from the borohydride-reduced gold colloids were able to improve the SPR signal response by 13 times higher than SPR signal produced without the enhancement species on the CM5 surface. The signal enhancement on the SAM surface using the same enhancement species was even greater at 29 times higher. The sensitivity of the assay was, however, unable to be determined due to time constraint. The limit of detection (LOD) of the progesterone assay using the CM5 chip was estimated to be ca. 5-20 pg/mL. Whilst for the SAM chip, the LOD of the progesterone assay was estimated to be ca. 5-20 fg/mL. Further work is required to confirm these estimated LOD values.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherThe University of Waikatoen_NZ
dc.rightsAll items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectgold colloidsen_NZ
dc.subjectsurface plasmon resonanceen_NZ
dc.subjectarabinogalactanen_NZ
dc.subjectsensitivity enhancementen_NZ
dc.titlePreparation of Stable Gold Colloids for Sensitivity Enhancement of Progesterone Immunoassay using Surface Plasmon Resonanceen_NZ
dc.typeThesisen_NZ
thesis.degree.disciplineScience and Engineeringen_NZ
thesis.degree.grantorUniversity of Waikatoen_NZ
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (MSc)en_NZ
uow.date.accession2007-02-23T23:23:38Zen_NZ
uow.date.available2009-06-12T01:01:01Zen_NZ
uow.identifier.adthttp://adt.waikato.ac.nz/public/adt-uow20070223.004336en_NZ
uow.date.migrated2009-07-08T23:23:38Zen_NZ
pubs.place-of-publicationHamilton, New Zealanden_NZ


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