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dc.contributor.authorLoader, Jared I.
dc.contributor.authorHawkes, Allan D.
dc.contributor.authorBeuzenberg, Veronica
dc.contributor.authorJensen, Dwayne J.
dc.contributor.authorCooney, Janine M.
dc.contributor.authorWilkins, Alistair L.
dc.contributor.authorFitzgerald, Joan M.
dc.contributor.authorBriggs, Lyn R.
dc.contributor.authorMiles, Christopher O.
dc.date.accessioned2009-08-18T22:26:25Z
dc.date.available2009-08-18T22:26:25Z
dc.date.issued2007
dc.identifier.citationLoader, J. I., Hawkes, A. D., Beuzenberg, V., et al. (2007). Convenient large-scale purification of yessotoxin from Protoceratium reticulatum culture and isolation of a novel furanoyessotoxin. Journal of Agricultural and Food Chemistry, 55(26), 11093-11100.en
dc.identifier.urihttps://hdl.handle.net/10289/2844
dc.description.abstractYessotoxins from a large-scale culture (226 L) of Protoceratium reticulatum strain CAWD129 were harvested by filtration followed by solid-phase extraction. The extract was purified by column chromatography over basic alumina and reverse-phase flash chromatography to afford pure yessotoxin (193 mg). Isolation of yessotoxin was greatly facilitated by selection of a strain which did not produce analogues that interfered with yessotoxin isolation. In addition to yessotoxin, numerous minor yessotoxins were detected by LC-MS in other fractions. From one of these, an early eluting minor analogue with the same molecular weight as yessotoxin and a similar mass spectrometric fragmentation pattern was isolated. This analogue was identified by NMR and mass spectrometry as a novel yessotoxin analogue containing a furan ring in the side chain. This finding reveals biosynthetic flexibility of the yessotoxin pathway in P. reticulatum and confirms earlier findings of production of many minor yessotoxin analogues by this alga. Production of these analogues appeared to be a constitutive trait of P. reticulatum CAWD129.en
dc.language.isoen
dc.publisherAmerican Chemical Societyen_NZ
dc.relation.urihttp://pubs.acs.org/doi/abs/10.1021/jf072704zen
dc.subjectchemistryen
dc.subjectProtoceratium reticulatumen
dc.titleConvenient large-scale purification of yessotoxin from Protoceratium reticulatum culture and isolation of a novel furanoyessotoxinen
dc.typeJournal Articleen
dc.identifier.doi10.1021/jf072704zen
dc.relation.isPartOfJournal of Agricultural and Food Chemistryen_NZ
pubs.begin-page11093en_NZ
pubs.elements-id34272
pubs.end-page11100en_NZ
pubs.issue26en_NZ
pubs.volume55en_NZ


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