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      Development of a Method of Analysis by Gas Chromatography-Mass Spectrometry using Selected Ion Monitoring for the Measurement of Fish Sex Steroids in Water

      Boult, Cherie Helen
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      Boult, C. H. (2009). Development of a Method of Analysis by Gas Chromatography-Mass Spectrometry using Selected Ion Monitoring for the Measurement of Fish Sex Steroids in Water (Thesis). The University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/3581
      Permanent Research Commons link: https://hdl.handle.net/10289/3581
      Abstract
      A method for the analysis of testosterone, androstenedione, 5β-dihydrotestosterone, 11-ketotestosterone, estrone, 17β-estradiol, 17α-hydroxyprogesterone, 17α,20β-dihydroxy-4-pregnen-3-one, 5β-pregnane-3α,17α,20α-triol, 5β-androstane-3α,17β-diol, 5β-androstane-3β,17β-diol, 5β-pregnane-3α,17α,20β-triol, 5β-androstane-3α,11β-diol-17-one, 5β-pregnane-3α,17α-diol-20-one, 17α,20β,21-trihydroxy-pregn-4-en-3-one and 11-deoxycortisol in water was developed using gas chromatography-mass spectrometry with selected ion monitoring.

      Fish sex steroids were extracted from holding water using C18 SPE cartridges, and eluted using methanol. Free and glucuronide conjugate levels of steroids were determined, glucuronide conjugates by hydrolysis using β-glucuronidase prior to

      derivatisation and free steroids by direct derivatisation.

      The method thus developed was applied to twelve goldfish holding water samples.

      Androstenedione was found to be excreted in considerably higher quantities by

      female ovulatory goldfish than spermiating goldfish, in contrast to previous

      research in this area.
      Date
      2009
      Type
      Thesis
      Publisher
      The University of Waikato
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