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dc.contributor.authorWu, Po-Hung
dc.contributor.authorNair, Giridhar R.
dc.contributor.authorChu, I-Ming
dc.contributor.authorWu, Wen-Teng
dc.date.accessioned2010-07-18T22:48:53Z
dc.date.available2010-07-18T22:48:53Z
dc.date.issued2008
dc.identifier.citationWu, P.-H., Nair, G.R., Chu, I-M., Wu, W.-T. (2008). High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis. Journal of Industrial Microbiology & Biotechnology, 35(2), 95-101.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4156
dc.description.abstractA fed-batch culture strategy for the production of recombinant Escherichia coli cells anchoring surface-displayed transglucosidase for use as a whole-cell biocatalyst for α-arbutin synthesis was developed. Lactose was used as an inducer of the recombinant protein. In fed-batch cultures, dissolved oxygen was used as the feed indicator for glucose, thus accumulation of glucose and acetate that affected the cell growth and recombinant protein production was avoided. Fed-batch fermentation with lactose induction yielded a biomass of 18 g/L, and the cells possessed very high transglucosylation activity. In the synthesis of α-arbutin by hydroquinone glucosylation, the whole-cell biocatalysts showed a specific activity of 501 nkat/g cell and produced 21 g/L of arbutin, which corresponded to 76% molar conversion. A sixfold increased productivity of whole cell biocatalysts was obtained in the fed-batch culture with lactose induction, as compared to batch culture induced by IPTG.en_NZ
dc.language.isoen
dc.publisherSpringeren_NZ
dc.relation.urihttp://www.springerlink.com/content/y31637h8553jp2q1/en_NZ
dc.subjectEscherichia colien_NZ
dc.subjectsurface-displayen_NZ
dc.subjecttransglucosidaseen_NZ
dc.subjectinductionen_NZ
dc.subjectfed-batchen_NZ
dc.titleHigh cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesisen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1007/s10295-007-0270-0en_NZ
dc.relation.isPartOfJournal of Industrial Microbiology and Biotechnologyen_NZ
pubs.begin-page95en_NZ
pubs.elements-id34111
pubs.end-page101en_NZ
pubs.issue2en_NZ
pubs.volume35en_NZ


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