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dc.contributor.authorReat, Valerie
dc.contributor.authorFinney, John L.
dc.contributor.authorSteer, Andrew
dc.contributor.authorRoberts, Mark A.
dc.contributor.authorSmith, Jeremy C.
dc.contributor.authorDunn, Rachel V.
dc.contributor.authorPeterson, Michelle E.
dc.contributor.authorDaniel, Roy M.
dc.date.accessioned2010-08-30T00:56:37Z
dc.date.available2010-08-30T00:56:37Z
dc.date.issued2000
dc.identifier.citationReat, V., Finney, J.L., Steer, A., Roberts, M.A., Smith, J., …, Daniel, R. M. (2000). Cryosolvents useful for protein and enzyme studies below −100°C. Journal of Biochemical and Biophysical Methods, 42(3), 97-103.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4461
dc.description.abstractFor the study of protein structure, dynamics, and function, at very low temperatures it is desirable to use cryosolvents that resist phase separation and crystallisation. We have examined these properties in a variety of cryosolvents. Using visual and X-ray diffraction criteria, methanol:ethanediol (70%:10%), methanol:glycerol (70%:10%), acetone:methoxyethanol:ethanediol (35%:35%:10%), dimethylformamide:ethanediol (70%:10%), dimethylformamide (80%), methoxyethanol (80%), and methoxyethanol:ethanediol (70%:10%) were all found to be free of phase-changes down to at least −160°C. The least viscous of these, methanol:ethanediol (70%:10%), was miscible down to −125°C and showed no exo or endothermic transitions when examined using DSC. It is therefore potentially particularly suitable for very low temperature cryoenzymology.en_NZ
dc.language.isoen
dc.publisherElsevieren_NZ
dc.subjectcryosolventen_NZ
dc.subjectcryoenzymologyen_NZ
dc.subjectmethanolen_NZ
dc.subjectphase changeen_NZ
dc.subjectX-ray diffractionen_NZ
dc.subjectDSCen_NZ
dc.titleCryosolvents useful for protein and enzyme studies below −100°Cen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1016/S0165-022X(99)00053-6en_NZ
dc.relation.isPartOfJournal of Biochemical and Biophysical Methodsen_NZ
pubs.begin-page97en_NZ
pubs.elements-id41265
pubs.end-page103en_NZ
pubs.volumeMethods 42en_NZ


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