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      Citrate synthases from the Archaea: Development of a bio-specific, affinity chromatography purification procedure

      James, Keith D.; Russell, Rupert J.M.; Parker, Lynne; Daniel, Roy M.; Hough, David W.; Danson, Michael J.
      DOI
       10.1111/j.1574-6968.1994.tb06886.x
      Link
       onlinelibrary.wiley.com
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      Citation
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      James, K.D., Russell, R.J.M., Parker, L., Daniel, R.M., Hough, D.W. & Danson, M.J. (1994). Citrate synthases from the Archaea: Development of a bio-specific, affinity chromatography purification procedure. FEMS Microbiology Letters, 119(1-2), 181-185.
      Permanent Research Commons link: https://hdl.handle.net/10289/4478
      Abstract
      Citrate synthases from both thermophilic and halophilic Archaea have been purified to homogeneity using affinity chromatography on Matrex Gel Red A and elution with a combination of substrate (oxaloacetate) and product (coenzyme A). In a number of cases, purification from cell-extract to protein suitable for N-terminal sequencing can be achieved by this single-step procedure. The method is particularly useful in the rapid purification of a thermophilic archaeal citrate synthase from a cloned gene expressed in a mesophilic host.
      Date
      1994
      Type
      Journal Article
      Publisher
      Wiley
      Collections
      • Science and Engineering Papers [3124]
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