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dc.contributor.authorJames, Keith D.
dc.contributor.authorRussell, Rupert J.M.
dc.contributor.authorParker, Lynne
dc.contributor.authorDaniel, Roy M.
dc.contributor.authorHough, David W.
dc.contributor.authorDanson, Michael J.
dc.date.accessioned2010-08-30T23:59:03Z
dc.date.available2010-08-30T23:59:03Z
dc.date.issued1994
dc.identifier.citationJames, K.D., Russell, R.J.M., Parker, L., Daniel, R.M., Hough, D.W. & Danson, M.J. (1994). Citrate synthases from the Archaea: Development of a bio-specific, affinity chromatography purification procedure. FEMS Microbiology Letters, 119(1-2), 181-185.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4478
dc.description.abstractCitrate synthases from both thermophilic and halophilic Archaea have been purified to homogeneity using affinity chromatography on Matrex Gel Red A and elution with a combination of substrate (oxaloacetate) and product (coenzyme A). In a number of cases, purification from cell-extract to protein suitable for N-terminal sequencing can be achieved by this single-step procedure. The method is particularly useful in the rapid purification of a thermophilic archaeal citrate synthase from a cloned gene expressed in a mesophilic host.en_NZ
dc.language.isoen
dc.publisherWileyen_NZ
dc.relation.urihttp://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1994.tb06886.x/abstracten_NZ
dc.subjectarchaeaen_NZ
dc.subjectthermophileen_NZ
dc.subjecthalophileen_NZ
dc.subjectcitrate synthase affinity chromatographyen_NZ
dc.titleCitrate synthases from the Archaea: Development of a bio-specific, affinity chromatography purification procedureen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1111/j.1574-6968.1994.tb06886.xen_NZ


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