Purification of a Thermostable DNA Polymerase from a Thermotoga Species
Simpson, H.D., Coolbear, T. & Daniel, R.M. (1990). Purification of a Thermostable DNA Polymerase from a Thermotoga Species. Annuals of the New York Academy of Sciences, 613, 426-428.
Permanent Research Commons link: https://hdl.handle.net/10289/4503
Most of the initial studies on the fundamental cellular process of biosynthesis and repair of deoxyribonucleic acid (DNA) have been carried out on the bacterium Escherichia coli.¹When a single-stranded DNA template is provided with a primer, the 5’ - 3’ polymerase copies the template in the presence of deoxyribonucleotide triphosphates and a divalent cation, for example, Mg²⁺.