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Permanent Research Commons link: https://hdl.handle.net/10289/4503
Most of the initial studies on the fundamental cellular process of biosynthesis and repair of deoxyribonucleic acid (DNA) have been carried out on the bacterium Escherichia coli.¹When a single-stranded DNA template is provided with a primer, the 5’ - 3’ polymerase copies the template in the presence of deoxyribonucleotide triphosphates and a divalent cation, for example, Mg²⁺.