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dc.contributor.authorWest, P.A.
dc.contributor.authorDaniel, Roy M.
dc.contributor.authorKnowles, C.J.
dc.contributor.authorLee, J.V.
dc.identifier.citationWest, P.A., Daniel, R.M., Knowles, C.J. & Lee, J.V. (1978). Tetramethyl-p-phenylenediamine (TMPD) oxidase activity and cytochrome distribution in the genus. FEMS Microbiology Letters, 4(6), 339-342.en_NZ
dc.description.abstractKovacs' [1] qualitative test for N,N,N',N',-tetramethyl- p-phenylenediamine (TMPD) oxidase activity is a fundamental test in the identification of bacteria [2,3]. TMPD (E~ = +0.26 V at pH 7.0) acts as an artificial electron donor to the terminal portion of aerobic respiratory systems [4], and the ability to oxidise TMPD, reduced by ascorbate, has been widely used as a quantitative assay of terminal oxidase activity [5]. In a wide range of heterotrophic bacteria there is a close correlation between their response to the Kovacs test and their ascorbate-TMPD oxidase activity measured manometrically [6]. The inability to oxidise ascorbate-TMPD or a negative response to the Kovacs test, however, does not necessarily indicate that an organism lacks an aerobic respiratory system and a terminal oxidase. For example, species belonging to the family Enterobacteriaceae are oxidase- negative yet form aerobic respiratory systems[5,7].en_NZ
dc.titleTetramethyl-p-phenylenediamine (TMPD) oxidase activity and cytochrome distribution in the genusen_NZ
dc.typeJournal Articleen_NZ

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