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      Determination of total potentially available nucleosides in bovine milk

      Gill, Brendon D.; Indyk, Harvey E.; Manley-Harris, Merilyn
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      Determination of total potentially.pdf
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      DOI
       10.1016/j.idairyj.2010.07.004
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      Gill, B.D., Indyk, H.E. & Manley-Harris, M. (2010). Determination of total potentially available nucleosides in bovine milk. International Dairy Journal, 21(1), 34-41.
      Permanent Research Commons link: https://hdl.handle.net/10289/4617
      Abstract
      Bovine colostrum and milk samples were collected from two herds over the course of the first month post-partum, pooled for each herd by stage of lactation and total potentially available nucleosides were determined. Sample analysis consisted of parallel enzymatic treatments, phenylboronate clean-up, and liquid chromatography to quantify contributions of nucleosides, monomeric nucleotides, nucleotide adducts, and polymeric nucleotides to the available nucleosides pool. Bovine colostrum contained high levels of nucleosides and monomeric nucleotides, which rapidly decreased as lactation progressed into transitional milk. Mature milk was relatively consistent in nucleoside and monomeric nucleotide concentrations from approximately the tenth day post-partum. Differences in concentrations between summer-milk and winter-milk herds were largely attributable to variability in uridine and monomeric nucleotide concentrations.
      Date
      2010
      Type
      Journal Article
      Publisher
      Elsevier
      Rights
      This is an author’s accepted version of an article published in the journal: International Dairy Journal. © 2010 Elsevier.
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