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dc.contributor.authorTalele, Sadhana
dc.contributor.authorGaynor, Paul
dc.contributor.authorvan Ekeran, Jethro
dc.contributor.authorCree, Michael J.
dc.date.accessioned2010-09-30T02:22:39Z
dc.date.available2010-09-30T02:22:39Z
dc.date.issued2010
dc.identifier.citationTalele, S., Gaynor, P., van Ekeran, J. & Cree, M.J. (2010). Modelling single cell electroporation with bipolar pulse: Simulating dependance of electroporated fractional pore area on the bipolar field frequency. In M. Iskander et al. (eds.), Technological developments in education and automation (pp. 355-359). Springer.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/4625
dc.description.abstractElectroporation EP, in which external electric field pulses create transient pores in a cell membrane, is an important technique for delivery of DNA and drugs into the cell. To enable entry of DNA into cells, the pores should have sufficiently large radii, remain open long enough for the DNA chain to enter the cell, and should not cause membrane rupture. A numerical model for a single spherical cell electroporated by application of direct and/or alternating external electric field pulses has been developed. The model is used to calculate the transmembrane potential, the number of pores and the the fraction of area occupied by the pores (fractional pore area FPA) in response to the various electric field pulses. Presented here are simulation results used to compare the extent of electroporation (fractional pore area FPA) in response to eletric field pulses of different frequencies in a range of extracellular conductivity for two cell raii. It is also observed that a 1 MHz bipolar sinusoidal applied electric field pulse reduces the relative difference in fractional pore area between the two cell sizes compared to a 100 kHz pulse.en_NZ
dc.language.isoen
dc.publisherSpringerLinken_NZ
dc.relation.urihttp://www.springerlink.com/content/k03836m42k64x508/en_NZ
dc.subjectelectroporationen_NZ
dc.subjectAC pulsesen_NZ
dc.subjectpore densityen_NZ
dc.subjectnumerical modelen_NZ
dc.subjectfractional pore areaen_NZ
dc.titleModelling single cell electroporation with bipolar pulse: Simulating dependance of electroporated fractional pore area on the bipolar field frequencyen_NZ
dc.typeChapter in Booken_NZ
dc.identifier.doi10.1007/978-90-481-3656-8_65en_NZ
dc.relation.isPartOfTechnological Developments in Education and Automationen_NZ
pubs.begin-page355en_NZ
pubs.elements-id9306
pubs.end-page359en_NZ


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