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dc.contributor.authorClark, Alan J.
dc.contributor.authorBanks, Jonathan C.
dc.contributor.authorWaas, Joseph R.
dc.date.accessioned2013-02-26T20:33:00Z
dc.date.available2013-02-26T20:33:00Z
dc.date.issued2013
dc.identifier.citationClark, A.J., Banks, J.C., & Waas, J.R. (2012). Economical genotyping of little blue penguin (Eudyptula minor) clades from feather-based DNA. New Zealand Journal of Ecology, published online 26 September 2012.en_NZ
dc.identifier.issn0110-6465
dc.identifier.urihttps://hdl.handle.net/10289/7264
dc.description.abstractDetermination of clade membership is a crucial requirement for many research questions addressing phylogeography, population structure, mating patterns, speciation, and hybridisation. The little blue penguin (Eudyptula minor) can be separated into two deeply divergent clades. However, assigning clade membership in little blue penguins requires molecular methods. Genetic sequencing can be used to identify clade membership but is expensive. Here, we present an economical alternative to the use of sequencing to determine little blue penguin clade membership. We extracted DNA from feathers using a method that produced reasonable quantities of DNA. We then amplified the D-loop section of the mitochondrial control region from total genomic DNA extracts, using the primers 'C L-tRNAglu' and 'D H-Dbox' followed by digestion with the restriction enzyme AluI. When visualised on a gel, distinctive banding patterns clearly indicated clade membership. We sequenced a subset of our samples and verified the accuracy of this method. The methods we present should facilitate little blue penguin research through a cost-effective approach to clade analysis as well as a successful technique to extract DNA from feathers when blood or tissue samples are not available.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherNew Zealand Ecological Society.en_NZ
dc.relation.urihttp://www.nzes.org.nz/nzje/en_NZ
dc.rights© New Zealand Ecology Society. Used with permission.en_NZ
dc.subjectCladesen_NZ
dc.subjectDNA extractionen_NZ
dc.subjectEnzymesen_NZ
dc.subjectFeathersen_NZ
dc.subjectRestriction RFLPen_NZ
dc.titleEconomical genotyping of little blue penguin (Eudyptula minor) clades from feather-based DNAen_NZ
dc.typeJournal Articleen_NZ
dc.relation.isPartOfNew Zealand Journal of Ecologyen_NZ
pubs.begin-page146en_NZ
pubs.elements-id38362
pubs.end-page150en_NZ
pubs.issue1en_NZ
pubs.volume37en_NZ


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