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dc.contributor.authorVoss, Logan J.
dc.contributor.authorVoss, Janet M.
dc.contributor.authorMcLeay, Lance M.
dc.contributor.authorSleigh, James W.
dc.date.accessioned2013-12-12T01:49:56Z
dc.date.available2013-12-12T01:49:56Z
dc.date.copyright2008-04
dc.date.issued2008
dc.identifier.citationVoss, L. J., Voss, J. M., McLeay, L., & Sleigh, J. W. (2008). Aconitine induces prolonged seizure-like events in rat neocortical brain slices. European Journal of Pharmacology, 584(2-3), 291-296.en_NZ
dc.identifier.urihttps://hdl.handle.net/10289/8333
dc.description.abstractAconitine effects on the hippocampal slice preparation have been well documented and include acute inhibitory and prolonged excitatory effects. To date, the effect of aconitine on neocortical activity has not been investigated. The aim of this study was to characterise the acute and long term effects of aconitine on cortical local field potential activity. Neocortical slices from juvenile Sprague–Dawley rats were perfused at room temperature with aconitine in normal artificial cerebrospinal fluid (aCSF) (n = 10). Spontaneous local field potential activity was recorded from the somatosensory cortex. The calcium dependence of aconitine-induced activity was investigated using low-calcium aCSF (n = 8). To isolate sodium and N-methyl-d-aspartate (NMDA) channel effects, phenytoin (n = 4) and 2-amino-5-phosphono-pentanoic acid (APV) (n = 6) were co-administered with aconitine, respectively. Aconitine consistently induced a dramatic increase in population “spike” activity after prolonged (89.5 ± 36.6 min) application in normal aCSF. This activity surge was eliminated in low-calcium aCSF and when aconitine was co-administered with phenytoin and APV. The acute effects of aconitine application were variable and included an increase in the frequency of population spikes, appearance of oscillatory seizure-like activity and prolonged bursts of multiunit activity. No acute inhibitory effects were observed. Aconitine has acute and prolonged excitatory effects on neocortical activity. The latter is effected by calcium-dependent mechanisms, in keeping with known effects of aconitine on hippocampal slices. Both sodium and NMDA channels are involved in mediating the calcium-dependent aconitine effects.en_NZ
dc.language.isoenen_NZ
dc.publisherElsevier BVen_NZ
dc.relation.ispartofEuropean Journal of Pharmacology
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0014299908001738en_NZ
dc.subjectcerebral cortexen_NZ
dc.subjectaconitineen_NZ
dc.subjectseizureen_NZ
dc.subjectcortical sliceen_NZ
dc.subject(Rat)en_NZ
dc.titleAconitine induces prolonged seizure-like events in rat neocortical brain slicesen_NZ
dc.typeJournal Articleen_NZ
dc.identifier.doi10.1016/j.ejphar.2008.02.024en_NZ
dc.relation.isPartOfEuropean Journal of Pharmacologyen_NZ
pubs.begin-page291en_NZ
pubs.elements-id33821
pubs.end-page296en_NZ
pubs.issue2-3en_NZ
pubs.volume584en_NZ


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