Show simple item record  

dc.contributor.authorNg, Timothy
dc.contributor.authorLay, Mark C.
dc.contributor.authorSwan, Janis E.
dc.coverage.spatialConference held in Perth, Western Australia
dc.date.accessioned2015-01-09T02:29:22Z
dc.date.available2014
dc.date.available2015-01-09T02:29:22Z
dc.date.issued2014
dc.identifier.citationNg, T., Lay, M. C., & Swan, J. E. (2014). Two phase aqueous extraction of whey proteins in a polyethylene glycol - arabinogalactan system. Presented at the CHEMECA 2014, Conference held in Perth, Western Australia, 28 September - 1 October 2014.en
dc.identifier.urihttps://hdl.handle.net/10289/9038
dc.description.abstractThe whey protein separation potential of aqueous two-phase systems of arabinogalactan [AG] (Lonza FiberAidTM) and polyethylene glycol [PEG], buffered with 10 mmol/g phosphate or citrate buffer, was studied. 100 mmol/g potassium chloride [KCl] was added as required. Previously-published phase equilibrium results were verified and the absorbance of whey protein isolate [WPI] in an AG-PEG solution was measured. The effect of pH, KCl concentration, initial WPI concentrations and upper to lower phase mass ratios on whey partitioning was studied. The best separation system contained 17.20% (w/w) AG, 7.20% (w/w) PEG, 10 mmol citrate buffer (pH 5.4) and 100 mmol KCl per gram of total system. The upper to lower phase mass and volume ratios were 1:1 and 16:11 respectively. Approximately 12 mg (mainly α-lactalbumin) of the 20 mg WPI added partitioned into the AG-rich upper phase. This system has potential to reduce chromatographic requirements in large scale separation of protein mixtures.
dc.format.extent1 - 15
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.relation.urihttp://www.icheme.org/~/media/Documents/Conferences/CHEMECA/Chemeca%202014/full-papers/1319_Two%20phase%20aqueous%20extraction%20of%20whey%20proteins%20in%20a%20polyethylene%20glycol-arabinogalactan%20system%20--%20revised.pdf
dc.sourceCHEMECA 2014
dc.subjecttwo-phase aqueous extraction
dc.subjectwhey
dc.subjectarabinogalactan
dc.subjectpolyethylene glycol
dc.titleTwo phase aqueous extraction of whey proteins in a polyethylene glycol - arabinogalactan system
dc.typeConference Contribution
dc.relation.isPartOfCHEMECA 2014en_NZ
pubs.begin-page1en_NZ
pubs.elements-id117762
pubs.end-page15en_NZ
pubs.finish-date2014-10-01
pubs.start-date2014-09-28


Files in this item

This item appears in the following Collection(s)

Show simple item record