Research Commons
      • Browse 
        • Communities & Collections
        • Titles
        • Authors
        • By Issue Date
        • Subjects
        • Types
        • Series
      • Help 
        • About
        • Collection Policy
        • OA Mandate Guidelines
        • Guidelines FAQ
        • Contact Us
      • My Account 
        • Sign In
        • Register
      View Item 
      •   Research Commons
      • University of Waikato Theses
      • Masters Degree Theses
      • View Item
      •   Research Commons
      • University of Waikato Theses
      • Masters Degree Theses
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Identifying Host Species of Dactylanthus taylorii using DNA Barcoding

      Parker, Cassarndra Marie
      Thumbnail
      Files
      thesis.pdf
      1.688Mb
      Citation
      Export citation
      Parker, C. M. (2015). Identifying Host Species of Dactylanthus taylorii using DNA Barcoding (Thesis, Master of Science (Research) (MSc(Research))). University of Waikato, Hamilton, New Zealand. Retrieved from https://hdl.handle.net/10289/9613
      Permanent Research Commons link: https://hdl.handle.net/10289/9613
      Abstract
      The population of the endangered endemic holoparasitic plant, Dactylanthus taylorii Hook F. (Balanophoraceae), in Pureora Forest Park was selected as the trial population for developing a method to accurately identify host species using DNA barcoding methods. The marker used was psbA-trnH, and ten samples were able to be successfully identified using the protocol trialed. Of these samples, nine were identified as Pseudopanax arboreus, with one result of Podocarpus totara. The marker used (psbA-trnH) provided adequate levels of variation to discriminate between the host species sequenced in the pilot study. Further work will focus on refining the protocol and trialing a second region for greater resolution of the Pseudopanax species cluster. The method used is relatively simple to employ, and resulted in no mortality of host or tuber. The ability to accurately identify hosts paves the way for the creation of an accurate host list which reflects the true host range and preferences of Dactylanthus taylorii. Globally, few host lists are accurate, and this poses significant challenges for managers. Future work will expand upon this study, collecting host root samples from populations across New Zealand to determine host preferences.
      Date
      2015
      Type
      Thesis
      Degree Name
      Master of Science (Research) (MSc(Research))
      Supervisors
      Gemmill, Chrissen E.C.
      Holzapfel, Avi
      Publisher
      University of Waikato
      Rights
      All items in Research Commons are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
      Collections
      • Masters Degree Theses [2385]
      Show full item record  

      Usage

      Downloads, last 12 months
      65
       
       

      Usage Statistics

      For this itemFor all of Research Commons

      The University of Waikato - Te Whare Wānanga o WaikatoFeedback and RequestsCopyright and Legal Statement